中国体外生命支持2026，Vol.24Issue(4)：333-336,4.DOI:10.13498/j.cnki.chin.j.ecls.2026.04.11
基于体外膜氧合平台的大鼠低温假死-延时复苏动物模型的建立
Establishment of a Rat Hypothermia-induced Suspended Animation and Delayed Resuscitation Model Based on an Extra-corporeal Membrane Oxygenation Platform
摘要
Abstract
Objective To explore the feasibility of establishing a rat model of hypothermic suspended animation with delayed resuscitation based on an ECMO platform.Methods Six healthy male Sprague-Dawley(SD)rats(SPF grade)were selected.Following the standard ECMO rat model procedure,cannulation was performed,and a hemorrhagic shock model was established by bloodletting through the jugular vein.Subsequently,cold Custodiol® organ preservation solution(HTK solution)was continuously perfused through the arterial cannula to induce cardiac arrest and systemic cooling to 15℃rectal temperature.After 60 minutes of circulatory arrest,blood perfusion was restored,and ECMO support was initiated to rewarm the rats to the target temperature until spontaneous heart rate recovery.Results All six rats successfully recovered spontaneous heartbeat under ECMO support.During the hemorrhagic shock model establishment,the blood withdrawal volume was(9.8±1.7)mL.The time from HTK solution perfusion to circulatory arrest at 15℃was(17±4)minutes.The lowest temperature during circulatory arrest was maintained at(14.6±1.2)℃,and the temperature at ECMO discontinuation was(34.3±0.7)℃.Conclusion The animal model of hypothermic suspended animation with delayed resuscitation in rats based on the ECMO platform is safe and feasible,laying a foundation for further exploration of the protective mechanism of hypothermic suspended animation with delayed resus-citation on vital organs.关键词
体外膜氧合/低温假死/延时复苏/大鼠/小动物模型Key words
extracorporeal membrane oxygenation/hypothermia-induced suspended animation/delayed resuscitation/rats/animal models引用本文复制引用
唐卓,王治上,孙渤轩,黄鑫怡,刘宇..基于体外膜氧合平台的大鼠低温假死-延时复苏动物模型的建立[J].中国体外生命支持,2026,24(4):333-336,4.基金项目
辽宁省科技计划联合计划(2023JH2/101700107) （2023JH2/101700107）
