中国兽医杂志2026，Vol.62Issue(8)：88-94,7.DOI:10.20157/j.cnki.zgsyzz.2026.sf.042
广西部分地区鸽源鼠伤寒沙门菌的分离鉴定、耐药性及致病性研究
Isolation,Identification,Drug Resistance and Pathogenicity of Salmonella typhimurium from Pigeons in Some Areas of Guangxi
摘要
Abstract
To investigate the Salmonella serotypes prevalent in meat pigeon farms in some regions of Guangxi,as well as their antimicrobial resistance,pathogenicity,and associated resistance and virulence genes,22 liver tissue samples were collected from dead squabs suspected of Salmonella infection for pathogen isolation and identification.Antimicrobial susceptibility testing,resistance gene detection,virulence gene detection,and animal regression experiments were subsequently performed.The results showed that 22 strains of Salmonella typhimurium were isolated and identified from the 22 liver tissue samples.Antimicrobial susceptibility testing revealed that the isolates showed the highest resistance rates to tetracycline and doxycycline,both reaching 81.8%(18/22).Resistance gene detection showed that the isolates carried the β-lactam resistance gene blaTEM(100%,22/22),the sulfonamide resistance gene sul2(63.6%,14/22),and the tetracycline resistance gene tet(A)(77.3%,17/22).Virulence gene detection demonstrated that all isolates carried 12 virulence genes,with detection rates of 100%(22/22)for each gene.Animal regression experiments showed that the isolates caused mortality in meat pigeons after infection,and polymerase chain reaction(PCR)confirmed that the isolates were Salmonella typhimurium.These results indicate that Salmonella typhimurium is the dominant serotype circulating in pigeon flocks in some regions of Guangxi.The isolates exhibited the highest resistance to tetracycline antibiotics,carried multiple antimicrobial resistance and virulence genes,and showed strong pathogenicity,providing a reference for the prevention and control of pigeon salmonellosis.关键词
肉鸽/鼠伤寒沙门菌/分离鉴定/耐药性/致病性Key words
meat pigeon/Salmonella typhimurium/isolation and identification/drug resistance/pathogenicity分类
农业科技引用本文复制引用
黄运福,俸祥仁,彭婷,刘伟,何深宏,颜国庆,兰天艳,银慧慧,曾雪颜,姜源明,赵武,唐作顺..广西部分地区鸽源鼠伤寒沙门菌的分离鉴定、耐药性及致病性研究[J].中国兽医杂志,2026,62(8):88-94,7.基金项目
国家现代农业产业技术体系广西创新团队建设专项(nycytxgxcxtd-2024-19-3) （nycytxgxcxtd-2024-19-3）
广西畜牧产业科技先锋队(桂农科盟202509-01-2) （桂农科盟202509-01-2）
广西农业职业技术大学科研项目(XKJ2332,XKJ2417) （XKJ2332,XKJ2417）
广西基本科研业务费项目(桂科专项26-8,桂科专项25-14,桂科专项25-15) （桂科专项26-8,桂科专项25-14,桂科专项25-15）
广西兽医生物技术重点实验室自主研究课题(24-035-32-A-05) （24-035-32-A-05）
