遵义医科大学学报2026，Vol.49Issue(8)：820-831,12.
Ferrostatin-1 通过诱导小胶质细胞 M2 极化抑制急性一氧化碳中毒神经炎症
Ferrostatin-1 suppresses acute carbon monoxide poisoning-mediated neuroin-flammation by inducing M2 polarisation in microglia
摘要
Abstract
Objective To establish a mouse model of acute carbon monoxide poisoning(ACMP),to observe pathological changes in the cerebral cortex following poisoning,and to preliminarily investigate the effect of fer-rostatin-1(Fer-1)on M2 polarisation of microglia in ACMP mice.Methods An ACMP mouse model was estab-lished using acute static carbon monoxide(CO)inhalation.The experiment was divided into a Sham group,an ACMP group and an ACMP+Fer-1 group.In the intervention group,Fer-1(10 mg/kg)was administered by in-traperitoneal injection immediately after modelling,with continuous administration for 14 days;behavioural as-sessments and tissue sampling on day 14 were both carried out 24 hours after the final dose.Behavioural tests were conducted on mice in each group on days 1,3,7 and 14 following poisoning to assess changes in neurologi-cal function.Cortical tissue was collected from the mice at 72 hours post-poisoning,and the expression levels of M1/M2-type microglial markers were assessed via Western blotting and immunofluorescence.Results Behaviour-al results showed that,compared with the Sham group,the ACMP group exhibited a significant reduction in neu-rological function scores from day 3 onwards(P<0.01),which deteriorated further over time;whereas the AC-MP+Fer-1 group showed a marked improvement in neurological function scores compared with the ACMP group from day 3 onwards(P<0.01).Neuroinflammation-related analyses revealed that,on day 3 following intoxica-tion,compared with the Sham group,both the expression and fluorescence intensity of M1-type microglial marker proteins in the cerebral cortex of the ACMP group were significantly elevated(P<0.01);whereas in the ACMP+Fer-1 group,these parameters were significantly lower than those in the ACMP group(P<0.01).Concur-rently,the expression and fluorescence intensity of M2-type marker proteins in the ACMP group were higher than those in the Sham group(P<0.01),and these were further elevated in the ACMP+Fer-1 group compared with the ACMP group(P<0.01).Conclusion ACMP can activate microglia to mediate a neuroinflammatory re-sponse,leading to neurological dysfunction;Fer-1 may be involved in the polarisation of microglia towards the M2 phenotype,thereby alleviating neuroinflammation and promoting neurological recovery following ACMP.关键词
急性一氧化碳中毒/铁死亡抑制素-1/小胶质细胞极化/神经炎症Key words
acute carbon monoxide poisoning/ferrostatin-1/microglia polarisation/neuroinflammation分类
医药卫生引用本文复制引用
王树洪,田银,姜栩恒,刘安平,陈江华,吴瑾,陆元兰,喻安永..Ferrostatin-1 通过诱导小胶质细胞 M2 极化抑制急性一氧化碳中毒神经炎症[J].遵义医科大学学报,2026,49(8):820-831,12.基金项目
国家自然科学基金资助项目(NO:82260254) （NO:82260254）
遵义市科技计划项目[NO:遵市科合 HZ 字(2023)218] （2023）
北京协和睿E急诊医学研究基金资助项目(NO:PUMF01010010-2024-23). （NO:PUMF01010010-2024-23）
