遵义医科大学学报2026，Vol.49Issue(8)：848-856,9.
铜死亡信号通路 SLC31A1/FDX1/LIAS 在小鼠子宫内膜崩解脱落中的作用机制
Mechanism of the cuproptosis signaling pathway SLC31A1/FDX1/LIAS in mouse endometrial breakdown and shedding
摘要
Abstract
Objective To explore the mechanism of the cuproptosis signaling pathway SLC31A1/FDX1/LIAS in the disintegration and shedding of mouse endometrium.Methods Eighty mature C57BL/6J female mice under-went oophorectomy and were divided into two groups.The mice in the TTM group were given the copper ion che-lator tetrathiomolybdate(TTM)by gavage once a day,for 2 weeks,starting from the 5th day.While the control group mice were given an equal amount of physiological saline by gavage.All mice were sequentially injected with estrogen,progesterone,and sterile peanut oil into the uterine cavity to induce endometrial decidualization.Mice were euthanized by cervical dislocation at 0,8,12,16,24 h after progesterone withdrawal,and uterine horns were collected.HE staining was used to observe the histological changes of mouse uterus.Chemical colori-metric method was used to detect the content of Cu2+and malondialdehyde(MDA)in mouse uterine tissue.QRT-PCR and Western blot were used to detect the expression of SLC31A1,FDX1,LIAS mRNA and protein in mouse uterine tissue.Results Starting from 16 hours after progesterone withdrawal,the endometrium of mice gradually disintegrated and detached from the basal layer,while the disintegration range and bleeding degree of endometrium in TTM group mice were reduced.After 0-16 hours of progesterone withdrawal,the content of Cu2+and MDA in the uterine tissue of the control group mice increased significantly,while it decreased significantly after TTM administration.At 12 and 16 hours after progesterone withdrawal,the mRNA and protein expression levels of SLC31A1 and FDXA1 in the uterine tissue of the control group mice significantly increased,while the mRNA and protein expression of LIAS reached its peak at 16 hours.The mRNA and protein expression levels of SLC31A1,FDX1 and LIAS in the TTM group were significantly inhibited.Conclusion The breakdown and shed-ding of mouse endometrium may be related to an increase in Cu2+influx into decidualized endometrial stromal cells,which upregulates the expression of SLC31A1/FDX1/LIAS and induces cuproptosis in stromal cells.Mo-reover,cuproptosis in mouse endometrial stromal cells caused by progesterone withdrawal is inhibited by TTM ad-ministration.关键词
月经/小鼠/铜死亡/内膜崩解Key words
cuproptosis/mouse/menstruation/endometrial breakdown分类
医药卫生引用本文复制引用
朱美如,于明明,杨秋红,肖煜璇,王贵云,谢卓,王乾兴..铜死亡信号通路 SLC31A1/FDX1/LIAS 在小鼠子宫内膜崩解脱落中的作用机制[J].遵义医科大学学报,2026,49(8):848-856,9.基金项目
遵义市科学技术基金资助项目[NO:遵市科合社字(2018)24]. （2018）
