中国蔬菜Issue(8)：84-92,9.DOI:10.19928/j.cnki.1000-6346.2026.0033
白菜黑斑病人工接种鉴定体系的建立以及种质资源筛选
Establishment of Robust Artificial Inoculation System for Evaluating Resistance to Black Spot Disease and Screening of Chinese Cabbage Germplasm
摘要
Abstract
Black spot disease is a major fungal disease threatening Chinese cabbage production worldwide.To establish a reliable,reproducible,and efficient phenotyping system to evaluate seedling resistance to black spot disease caused by Alternaria brassicicola,we systematically optimized the inoculation conditions by comparing inoculation methods,spore suspension concentrations,and incubation temperatures.The results showed that detached-leaf inoculation performed on seedlings with a spore suspension of 1×105 spores·mL-1,followed by incubation at 25℃for 3 days,provided the most effective and repeatable conditions for assessing black spot disease resistance.The optimized assay was further validated using 53 elite Chinese cabbage inbred lines,with parallel field disease assessments conducted at the adult plant stage.The high concordance between the two evaluation approaches verified the accuracy,stability,and reliability of the artificial inoculation system.Furthermore,germplasm lines representing the extremes of disease resistance were selected,providing valuable parental materials for identifying genes associated with black spot disease resistance.Together,these results provide both germplasm resources and a technical foundation to advance molecular breeding for enhanced black spot disease resistance in Chinese cabbage.关键词
白菜/黑斑病/抗性评价/表型鉴定/人工接种Key words
Chinese cabbage/black spot disease/resistance evaluation/phenotype identification/artificial inoculation引用本文复制引用
张红,范伟强,刘晓晖,张斌,赵艳艳,苏悦华,黄志银,王超楠..白菜黑斑病人工接种鉴定体系的建立以及种质资源筛选[J].中国蔬菜,2026,(8):84-92,9.基金项目
国家重点研发计划子课题(2023YFD1201504-4),天津市科技重大专项与工程全国重点实验室重大专项(25ZXZSSS00050),天津市科技重大专项与工程种业创新重大专项(23ZXZVSN00030),国家大宗蔬菜产业技术体系综合试验站项目(CARS-23-G-05),天津市优秀农业科技特派员项目(25ZYCGSN00610) （2023YFD1201504-4）
