东南园艺2026，Vol.14Issue(3)：290-295,6.DOI:10.20023/j.cnki.2095-5774.2026.03.006
文心兰'红梦香'离体快繁技术研究
Study on in vitro rapid propagation technology of Oncidium Twinkle'Chian-Tzy Deilight'
摘要
Abstract
[Objective]To establish an efficient and stable in vitro cluster bud rapid propagation system for fragrant Oncidium Twinkle'Chian-Tzy Deilight',fill the technical gap in industrial seedling raising of this cultivar,and break through the two core bottlenecks of rapid propagation:proliferation and rooting.[Method]Multiple shoots induced from flower stalk axillary buds were used as experimental materials.L16(45)and L9(34)orthogonal designs were adopted to systematically investigate the effects of five factors(basic medium,6-BA,KT,NAA and sucrose)on multiple shoot proliferation,as well as four factors(basic medium,NAA,IBA and organic additives)on multiple shoot proliferation.so as to screen the optimal combination each factor.[Result]The priority order of factors multiple shoot proliferation was 6-BA＞basic medium＞NAA＞KT＞sucrose.The optimal medium for proliferation was 1/2MS+Hyponex No.1 1.5 g/L+6-BA 1.2 mg/L+KT 0.3 mg/L+NAA 0.15 mg/L+sucrose 30 g/L+agar 6.5 g/L,with an average proliferation coefficient of 5.77 after 45 days of culture.The optimal rooting medium was Hyponex No.1 3.0 g/L+NAA 0.1 mg/L+IBA 0.2 mg/L+potato powder 10 g/L+sucrose 30 g/L+agar 7 g/L+activated carbon 0.5 g/L.The rooting rate reached 100%after 75 days,with an average of 9.5 roots per plantlet and an average root length of 4.0 cm.[Conclusion]The cluster bud-based micropropagation technique enhances the asexual propagation efficiency of Oncidium Twinkle'Chian-Tzy Deilight',offering theoretical basis and technical models for culture medium optimization of fragrant congeners in the same hybrid series.关键词
文心兰/丛生芽/正交设计/增殖/生根Key words
Oncidium/clustered buds/orthogonal design/proliferation/rooting分类
农业科技引用本文复制引用
赖宁静,林榕燕,方能炎,叶秀仙,林兵..文心兰'红梦香'离体快繁技术研究[J].东南园艺,2026,14(3):290-295,6.基金项目
福建省属公益类科研院所基本科研专项(2023R1096) （2023R1096）
福建省财政花卉产业发展项目(闽林文[2023]107号) （闽林文[2023]107号）
福建省农业科学院特色花卉创新团队项目(CXTD2021010-2) （CXTD2021010-2）
福建省农业科学院示范基地项目(Sfjd2303) （Sfjd2303）
