法医学杂志2026，Vol.42Issue(3)：215-219,5.DOI:10.12116/j.issn.1004-5619.2026.460404
溺死浮游微生物DNA快速检测技术的成果转化及应用
Rapid Detection of Planktonic Microorganism DNA in Drowning:Technology Transfer and Application Implications
摘要
Abstract
Conventional diatom-based assays for drowning diagnosis present multiple inherent limita-tions,including prolonged testing durations,poor adaptability to field examination,compromised detecta-bility in aquatic environments with low diatom abundance,and reliance on a single type of forensic evidence.To address the above limitations,our team selected drowning-relevant planktonic microorga-nisms,including diatoms,cyanobacteria,and planktonic bacteria,as molecular biomarkers and estab-lished a rapid planktonic microorganism DNA detection system based on real-time quantitative poly-merase chain reaction(qPCR).Correspondingly,a vehicle-mounted field DNA detection workstation was developed to enable on-site testing.Technology transfer was facilitated through an integrated industry-academia-research collaborative model involving public security forensic research divisions and industrial enterprises.Meanwhile,system performance validation was implemented in accordance with industrial specifications for diatom identification and standardized evaluation criteria for qPCR instru-ments.The developed system enabled on-site detection to be completed within 1.5 h,with a limit of detection(LOD)of 0.000 1 ng.All core technical parameters of the instrument exceeded existing indus-trial standards,with the advantages of convenient sample collection,high sensitivity,strong specificity,rapid testing,and ease of operation.This system not only satisfies the practical demands of forensic scene investigations but also complements morphological analysis by establishing an integrated"molecu-lar biology+morphology"evidence framework.Furthermore,it provides a new strategy for the rapid diagnosis of drowning and offers valuable insights into the technology transfer achievements within the field of forensic science.关键词
法医学/科技成果转化/溺死/浮游微生物/DNA检测/实时荧光定量PCRKey words
forensic medicine/technology transfer/drowning/planktonic microorganisms/DNA detec-tion/real-time quantitative PCR分类
医药卫生引用本文复制引用
徐际超,徐曲毅,陈晓晖,苏秦,谷党恩,林添春,张健苗,韩亮,蔡鸣,赵建..溺死浮游微生物DNA快速检测技术的成果转化及应用[J].法医学杂志,2026,42(3):215-219,5.基金项目
国家自然科学基金资助项目(82371901) （82371901）
国家重点研发计划资助项目(2023YFC3303902) （2023YFC3303902）
