法医学杂志2026，Vol.42Issue(3)：232-239,8.DOI:10.12116/j.issn.1004-5619.2026.360401
全自动血液中乙醇分析仪的研制及在法庭科学中的应用
Development of a Fully Automated Blood Alcohol Analyzer and Its Application in Forensic Science
摘要
Abstract
Blood alcohol concentration detection has long relied on manual operations in the field of forensic identification,resulting in cumbersome workflows,low throughput,and substantial human er-ror.Such drawbacks make it increasingly difficult to meet the growing demands for case-handling effi-ciency and evidentiary quality.To address these issues,this study presents a fully automatic blood alco-hol analyzer(Puyu LSAP AI),developed in-house by integrating precision mechanical actuation,pneu-matic flow-limited pipetting,and intelligent task scheduling technologies,which automates and reengi-neers the conventional workflow to establish a fully automated protocol for blood alcohol concentration detection.In accordance with the latest national standard,the system demonstrated excellent linearity over the mass concentration range of 0.10-3.00 mg/mL,with correlation coefficients r≥0.997.The limit of de-tection(LOD)and limit of quantification(LOQ)were 0.05 mg/mL and 0.10 mg/mL,respectively.Paral-lel analyses of 20 whole-blood samples with varying viscosities yielded relative deviations consistently ranging from 1%to 5%,while relative standard deviations from repeatability tests were below 2.5%(n=6).No carryover contamination was observed during alternating injections of high-concentration samples(5 mg/mL)and blank specimens,and continuous high-throughput operation for 24 h was completed without mechanical or communication malfunctions.This analyzer has been deployed for routine fo-rensic blood alcohol concentration detection,achieving fully automated,unattended,closed-loop opera-tion throughout the entire analytical workflow.Its core technical components have been domestically developed,while the software and hardware architectures have been comprehensively decoupled to im-prove modularity and maintainability.The system meets national standards for precision,accuracy,and full-process digital compliance.It significantly improves the case-handling efficiency and evidentiary quality,offering technical support and a practical application paradigm for the intelligent and automated transformation of forensic science instrumentation.关键词
法医学/科技成果转化/血液乙醇浓度检测/顶空气相色谱法/实验室自动化Key words
forensic medicine/technology transfer/blood alcohol concentration analysis/headspace gas chro-matography/laboratory automation分类
医药卫生引用本文复制引用
唐磊,谢伟宏,卓成飞,徐琛,姚焕焕,李晓飞,张建伟,李阳,应剑波..全自动血液中乙醇分析仪的研制及在法庭科学中的应用[J].法医学杂志,2026,42(3):232-239,8.基金项目
公安部刑事技术"双十计划"重点攻关项目 （）
