南方农业学报2026，Vol.57Issue(7)：1957-1966,10.DOI:10.3969/j.issn.2095-1191.2026.07.001
新斑型黄缘巧瓢虫线粒体基因组全序列测定与分析
Complete sequence detection and analysis on mitochondrial genome of new spot-pattern morph of Oenopia sauzeti(Coleoptera:Coccinellidae)
摘要
Abstract
[Objective]To investigate the structural characteristics of the mitochondrial genome of new spot-pattern morph of Oenopia sauzeti,and to analyze its phylogenetic relationships,thereby providing fundamental information for the study and application of the genetic diversity of this natural enemy insect.[Method]The mitochondrial genome of new spot-pattern morph of Oenopia sauzeti was sequenced using the Illumina high-throughput sequencing platform.Gene an-notation and bioinformatics analysis were performed.Based on 13 protein-coding genes(PCGs),a phylogenetic tree was constructed using the maximum likelihood method to clarify its phylogenetic position within the family Coccinellidae.[Result]The complete mitogenome of the new spot-pattern morph of Oenopia sauzeti was 20394 bp in length,comprising 2 rRNA genes,22 tRNA genes,13 PCGs,and 2 non-coding control regions.It contained 6 intergenic spacers and 11 gene overlaps.The nucleotide composition of new spot-pattern morph of Oenopia sauzeti was A content=38.58%,T con-tent=40.67%,C content=9.05%,and G content=11.70%,with GC content of 20.75%and AT content of 79.25%,indi-cating a pronounced AT bias.Among the 13 PCGs,COX1 gene initiated with the codon TTG,while the remaining genes initiated with codon ATN(ATA,ATG,or ATT).Regarding stop codons,COX1,COX2,COX3,and ND6 gens termi-nated with an incomplete T;CYTB gene terminated with the complete TAG;and the remaining genes terminated with the complete TAA.Among the 22 tRNA genes,21 exhibited the typical cloverleaf secondary structure,except for trnS1 gene,which lacked the dihydrouridine(D)arm,a characteristic feature of insect mitochondrion mt-tRNAs.Phylogenetic analysis revealed that the new spot-pattern morph of Oenopia sauzeti new clustered within a highly supported clade(100%)alongside the known Oenopia sauzeti,strongly confirming at the molecular level that this new spot-pattern morph belonged to Oenopia sauzeti.Furthermore,Aiolocaria hexaspilota was identified as the sister group to Oenopia sauzeti and Oenopia formosana.[Conclusion]The complete mitogenome sequence and structure of the new spot-pattern morph of Oenopia sauzeti are consistent with the general characteristics of coccinellid mitochondrial genomes.Oenopia sauzeti exhibits a close phylogenetic relationship with Oenopia formosana and Aiolocaria hexaspilota.关键词
新斑型黄缘巧瓢虫/线粒体/基因组/系统发育Key words
new spot-pattern morph of Oenopia sauzeti/mitochondrion/genome/phylogenetic relationship分类
农业科技引用本文复制引用
陈浩帆,何星,李亚金,赵林,张利娟,张宏瑞..新斑型黄缘巧瓢虫线粒体基因组全序列测定与分析[J].南方农业学报,2026,57(7):1957-1966,10.基金项目
云南省科技厅科技计划项目(202304BT090032) （202304BT090032）
华坪县农民院士科技服务站项目(云科协联[2023]9号) Science and Technology Plan Project of Yunnan Department of Science and Technology(202304BT090032) （云科协联[2023]9号）
Sci-tech Service Station of Farmer Academician in Huaping(Yunkexielian[2023]9) （Yunkexielian[2023]9）
