解放军医学院学报2026，Vol.47Issue(5)：473-483,11.DOI:10.12435/j.issn.2095-5227.25122301
甲基丙烯酰化明胶/β-磷酸三钙/黑色素纳米颗粒复合水凝胶对小鼠胚胎成骨细胞促成骨和抗活性氧作用的影响
Effects of GelMA/β-tricalcium-phosphate/melanin nanoparticle composite hydrogel on osteogenesis promotion and reactive oxygen species scavenging in mouse embryonic osteoblasts
张鲁岳 1黄萌 2张敏 3王朝祥 1刘醒钊 1刘天驰 1王碧莹 4曹均凯4
作者信息
- 1. 解放军医学院,北京 100853||解放军总医院第一医学中心口腔科,北京 100853
- 2. 解放军总医院第六医学中心口腔科,北京 100048
- 3. 解放军总医院第八医学中心口腔科,北京 100091
- 4. 解放军总医院第一医学中心口腔科,北京 100853
- 折叠
摘要
Abstract
Background By integrating gelatin methacryloyl(GelMA)with osteoconductive β-tricalcium phosphate(β-TCP)via tissue engineering strategies,we developed an implantable scaffold designed for bone defect regions.This composite material holds promise for addressing the challenges associated with bone repair and functional reconstruction.Objective To synthesize a GelMA/β-TCP/melanin nanoparticle(MNP)composite hydrogel and investigate its biocompatibility,reactive oxygen species(ROS)-scavenging capacity,biological properties,and osteogenic differentiation potential in MC3T3-E1 cells.Methods Four types of hydrogels were prepared under 0.25%LAP(photoinitiator)and 405 nm light:GelMA,GelMA/β-TCP,GelMA/MNPs,and GelMA/β-TCP/MNPs,using 5%GelMA,1%β-TCP,and 1 mg/mL MNPs.The microstructures of freeze-dried samples were characterized by scanning electron microscopy.Extracts from the four hydrogel groups were collected,with complete culture medium serving as a blank control.Biocompatibility was assessed using CCK-8 assay,live/dead staining,and TUNEL staining;anti-ROS activity was evaluated with DCFH-DA staining;biological property regulation was determined via phalloidin staining,Ki-67 immunofluorescence,and scratch wound assay;and osteogenic potential was assessed by RUNX2 and OCN immunofluorescence staining.Results Freeze-dried GelMA/β-TCP/MNP hydrogel exhibited a rough,three-dimensional porous structure with an average pore size of(103.61±16.90)μm,favorable for cell adhesion and proliferation.Biocompatibility assays indicated no obvious cytotoxicity in any group.Incorporation of MNPs into GelMA significantly enhanced intracellular ROS-scavenging activity.Biological property evaluations showed increased cell adhesion(phalloidin staining),promoted cell migration(scratch assay),and enhanced cell proliferation(Ki-67)in the GelMA/β-TCP/MNP group.Osteogenesis-related staining revealed that GelMA/β-TCP/MNP hydrogel exhibited the strongest osteoinductive ability compared with the control and other material groups.Conclusion GelMA/β-TCP/MNP hydrogel demonstrates excellent biocompatibility and effective ROS-scavenging activity,and enhances cell adhesion,migration,and proliferation,promote osteogenic differentiation of MC3T3-E1 cells,indicating promising potential for bone tissue engineering applications.关键词
β-磷酸三钙/黑色素/水凝胶/活性氧/成骨Key words
β-tricalcium phosphate/melanin nanoparticles/hydrogel/reactive oxygen species/osteogenesis分类
医药卫生引用本文复制引用
张鲁岳,黄萌,张敏,王朝祥,刘醒钊,刘天驰,王碧莹,曹均凯..甲基丙烯酰化明胶/β-磷酸三钙/黑色素纳米颗粒复合水凝胶对小鼠胚胎成骨细胞促成骨和抗活性氧作用的影响[J].解放军医学院学报,2026,47(5):473-483,11.
