林业科学2026，Vol.62Issue(9)：152-164,13.DOI:10.11707/j.1001-7488.LYKX20260041
粉红溲疏组培再生优化及遗传转化体系构建
Optimization of Tissue Culture Regeneration and Construction of Genetic Transformation System of Deutzia rubens
摘要
Abstract
[Objective]On the basis of two previously established tissue culture regeneration systems of Deutzia species,the regeneration systems of Deutzia were screened and optimized,and a genetic transformation system was constructed with the optimal regeneration system as the recipient system in this study,aiming to provide technical support for molecular breeding of important landscape tree species in Deutzia,and a reference paradigm for the de novo domestication of woody plants.[Method]The tissue culture rooted plantlets of six wild species in Deutzia were used as research materials.The leaves,petioles and internodes from the 1st to 5th node along the morphological axis were taken as explants.In the tissue culture regeneration process,the genotype,explant type and maturity were screened to optimize the tissue regeneration system of Deutzia.Subsequently,the optimized system used as the recipient system to construct an Agrobacterium tumefaciens-mediated leaf-disc genetic transformation system,and simultaneously screen the optimal concentration of Timentin.[Result]Among the six tested wild Deutzia species,the genotype with the best regeneration was Deutzia rubens.The optimum explant for the tissue culture regeneration of D.rubens was the first expanded leaf of 30-day-old rooted plantlets,with a differentiation rate of 100%.The rooting rate of regenerated plantlets reached 100%on 1/2 MS medium supplemented with 0.5 mg·L-1 IBA,showing the best growth of plantlets and root systems.The rooted plantlets grew best in the acclimatization and transplantation substrate with perlite∶nutrient soil=3∶7.The maximum plant height was 15.68 cm after 30 days of acclimatization.A.tumefaciens successfully infected the leaves of D.rubens and regenerated plantlets.The differentiation rate reached the maximum of 83.33%at 50 mg·L-1 Timentin,and the genetic transformation efficiency was 16.7%.Conventional PCR detection verified that exogenous Ruby and NtGRF4-NtGIF1 genes were integrated into the genome of regenerated D.rubens plants,and the transgenic plants showed a visible red phenotype.[Conclusion]This study has established an efficient and stable tissue culture regeneration system of Deutzia represented by D.rubens,and an Agrobacterium-mediated leaf-disc genetic transformation system is for the first time constructed.The result fills the technical gap in genetic transformation of Deutzia,and provides core technical support for gene function analysis and targeted molecular improvement of this genus,as well as a practical reference for the utilization and de novo domestication breeding of wild woody plant resources.关键词
粉红溲疏/组培再生/叶盘法/遗传转化/从头驯化Key words
Deutzia rubens/tissue culture regeneration/leaf-disc method/genetic transformation/de novo domestication分类
农业科技引用本文复制引用
郝英男,张鑫,袁禹婷,屠雨欣,冯馨怡,张余周,王慧慧,吴普侠,付春祥,李进宇..粉红溲疏组培再生优化及遗传转化体系构建[J].林业科学,2026,62(9):152-164,13.基金项目
黄土高原植被恢复与土壤水文过程的互馈机理及调控策略项目(U24A20629) （U24A20629）
国家重点研发计划项目(2024YFF306503). （2024YFF306503）
