饲料研究2026，Vol.49Issue(14)：96-101,6.DOI:10.13557/j.cnki.issn1002-2813.2026.14.016
刺五加果实多成分提取工艺优化及抗氧化活性分析
Optimization of multi-component extraction from Acanthopanax senticosus fruits and analysis of antioxidant activity
摘要
Abstract
The experiment aimed to optimize the extraction process of multiple components from Acanthopanax senticosus fruits and evaluate their in vitro antioxidant activity.High-performance liquid chromatography(HPLC)was employed for component analysis.The analytic hierarchy process(AHP)combined with the entropy weight method(EWM)was used to establish a multi-index comprehensive scoring model.Coupled with response surface methodology,the extraction process of eight target components from Acanthopanax senticosus fruits was optimized,and the scavenging capacities of the extract obtained under the optimal conditions against 1,1-diphenyl-2-picrylhydrazyl(DPPH)and hydroxyl(·OH)radicals were investigated.The results showed that the optimal extraction conditions for the multiple components from Acanthopanax senticosus fruits were liquid-to-solid ratio of 14 mL/g,ethanol concentration of 61%,and ultrasonic extraction time of 58 min.Under these conditions,the average comprehensive score was 94.05%,which was highly consistent with the predicted value(94.06%).The extract prepared by the optimized process exhibited strong scavenging effects against both DPPH and·OH radicals,with the maximum scavenging rates of 85.64%and 90.63%,respectively.The study shows that the established extraction process in the experiment is stable and feasible,and the extract from Acanthopanax senticosus fruits possesses good in vitro antioxidant activity,and can be further developed and utilized as a natural antioxidant feed additive resource.关键词
刺五加果实/层次分析法-熵权法/响应面法/多成分提取/抗氧化活性/饲料添加剂Key words
Acanthopanax senticosus fruits/analytic hierarchy process-entropy weight method/response surface methodology/multi-component extraction/antioxidant activity/feed additive分类
农业科技引用本文复制引用
王怡蒙,秦航,黄晓阳,郭盛磊..刺五加果实多成分提取工艺优化及抗氧化活性分析[J].饲料研究,2026,49(14):96-101,6.基金项目
国家重点研发项目(项目编号:2021YFD1600901) （项目编号:2021YFD1600901）
黑龙江省博士后科研启动金项目(项目编号:LBH-Q21044) （项目编号:LBH-Q21044）
