生物技术进展2026，Vol.16Issue(4)：732-743,12.DOI:10.19586/j.2095-2341.2026.0025
多组学系统分析生物育种产品非期望效应的研究进展
Research Progress on Multi-omics Systematic Analysis of Unintended Effects in Biotechnology-derived Breeding Products
摘要
Abstract
With the accelerating commercialization of genetically modified(GM)and gene-edited(GE)crops within global agri-cultural trade,the accurate identification of potential unintended effects(UEs)in biotechnology-derived breeding products has become a central issue in biosafety assessment.For a long duration,the evaluation system has relied largely on the principle of"substantial equivalence",wherein targeted detection technologies have played a significant role in assessing conventional physi-cochemical indicators.However,traditional methods struggle to capture complex molecular variations.Moreover,conventional"bulk multi-omics"analysis tends to mask cellular heterogeneity,failing to detect rare variants and their intricate microenviron-mental interactions at the microscopic level.This review summarized recent advances in the application of multi-omics technolo-gies for evaluating unintended effects in biotech breeding products.It focused on the potential of emerging technologies such as single-cell omics and spatial omics in deciphering tissue-specific unintended effects.Furthermore,the article provided an in-depth analysis of how non-targeted multi-omics—encompassing genomics,transcriptomics,proteomics,and metabolomics—en-able the comprehensive profiling of multi-layered molecular variations through full-spectrum scanning.Finally,the article ex-plored the challenges and prospects of integrated multi-omics data analysis and artificial intelligence(AI)in future biosafety reg-ulatory frameworks.Therefore,a systems biology strategy integrating multi-dimensional omics data,combined with machine learning and artificial intelligence,represents a crucial trend toward achieving comprehensive and precise food safety and unin-tended effects assessment of biotech breeding products.关键词
生物育种产品/非期望效应/多组学/单细胞组学/空间组学/生物安全评价Key words
biotechnology-derived breeding products/unintended effects/multi-omics/single-cell omics/spatial omics/biosafety assessment分类
生物科学引用本文复制引用
高宇,韩天意,贺晓云,程楠,刘威辰,付伟,黄昆仑,陈红..多组学系统分析生物育种产品非期望效应的研究进展[J].生物技术进展,2026,16(4):732-743,12.基金项目
农业生物育种重大专项(2023ZD0406304). （2023ZD0406304）
