生物技术进展2026，Vol.16Issue(4)：744-755,12.DOI:10.19586/j.2095-2341.2026.0130
转基因与基因编辑产品检测方法研究进展
Research Progress on Detection Methods of Genetically Modified and Gene-edited Products
摘要
Abstract
With the continuous expansion in the commercialization scale of genetically modified(GM)crops and the rapid applica-tion of emerging biotechnologies such as gene editing,products derived from these novel biotechnologies have become increasingly complicated and diversified.Establishing sensitive,accurate and traceable detection methodologies and systems for such biotech products serves as an essential foundation for food safety assurance,labeling administration as well as regulatory law enforcement.The article systematically reviewed the research advances in the detection of GM and gene-edited products and other novel biotech-derived products in 2025,focusing on sample pretreatment,nucleic acid amplification assays(including quantitative real-time PCR and digital PCR),isothermal amplification,clustered regularly interspaced short palindromic repeats-Cas protein(CRISPR/Cas)-assisted detection,biosensing and immunoassays,high-throughput sequencing,alongside databases and regulatory support-ing systems.Overall,quantitative real-time PCR(qPCR)remains the core technique for quantitative analysis of genetically modi-fied ingredients;digital PCR(dPCR)exhibits prominent advantages in absolute quantification of low-copy target sequences and val-ue assignment of reference materials.Isothermal amplification and biosensing technologies keep facilitating the development of rapid and on-site detection.High-throughput sequencing provides technical support with superior resolution for the identification of gene-edited products.In the future,relevant detection technologies will further evolve toward the integrated application of multiple tech-niques,simultaneous identification of multi-target analytes,intelligent data analysis and compatibility with regulatory requirements.关键词
转基因产品/基因编辑产品/检测方法/CRISPR/Cas/高通量测序Key words
genetically modified products/gene-edited products/detection methods/CRISPR/Cas/high-throughput sequencing分类
生物科学引用本文复制引用
张华,张雨腾,裴欣瑶,杨立桃..转基因与基因编辑产品检测方法研究进展[J].生物技术进展,2026,16(4):744-755,12.基金项目
上海市科技创新行动计划项目(24DZ2201300 （）
24390713700). （）
