生物技术进展2026，Vol.16Issue(4)：756-764,9.DOI:10.19586/j.2095-2341.2026.0049
CRISPR/Cas系统在转基因作物检测中的应用和展望
Application and Prospect of CRISPR/Cas System in Genetically Modified Detection
摘要
Abstract
With the widespread cultivation of genetically modified crops,there is an urgent need for efficient and accurate detec-tion technologies to protect consumer rights and enable government regulation.Although PCR-based molecular detection tech-niques have been widely adopted,there remains scope for improvement in on-site rapid screening,simultaneous multi-target de-tection,and device portability.The clustered regularly interspaced short palindromic repeats-associated protein(CRISPR/Cas)system,with its unique and powerful cleavage activity,offers a breakthrough solution for developing simple,on-site detection technologies.Toward the application of the CRISPR/Cas system in genetically modified detection,this review summarized its re-cent advances and current implementations.It elaborated on and analyzed the technical principles and practical applications of molecular diagnostic platforms based on Cas9,Cas12,Cas13,and Cas14 proteins,including their advantages in enhancing de-tection sensitivity,enabling multi-target recognition,simplifying operational procedures,and promoting device portability.The paper also summarized the current limitations of these technologies and discussed future development directions,aiming to ad-vance the progress of rapid,precise,and portable novel detection technologies.关键词
CRISPR/Cas/检测技术/转基因作物Key words
CRISPR/Cas/detection technology/genetically modified crops分类
生物科学引用本文复制引用
周佳奕,朱梨梨,何龚雯,姜大刚..CRISPR/Cas系统在转基因作物检测中的应用和展望[J].生物技术进展,2026,16(4):756-764,9.基金项目
农业生物育种重大专项(2023ZD04062). （2023ZD04062）
