生物技术进展2026，Vol.16Issue(4)：878-884,7.DOI:10.19586/j.2095-2341.2025.0077
CRISPR/Cas9基因编辑高油酸大豆快速检测方法研究
Study on Rapid Detection Method for CRISPR/Cas9-mediated Gene-edited High-oleic Soybean
摘要
Abstract
Gene editing,as an important and emerging cutting-edge biotechnology,has been widely applied in fields,such as life science research,species improvement,and human health.Currently,more than ten species of animals and plants have been approved for commercialization.The detection technology of gene-edited plants is the core support for controlling biosafety and regulating the seed industry market.Due to different regulatory requirements,detection methods and a standardized and com-plete monitoring system are crucial.Based on this,for clustered regularly interspaced short palindromic repeats/CRISPR-associ-ated protein 9(CRISPR/Cas9)-mediated gene-edited high-oleic soybean,multiple sets of specific primer probe sequences were designed by comparing their gene sequences with wild-type soybean.A set of primer probe sequences that met the requirements was screened using fluorescence PCR method,with a detection sensitivity of up to 0.01%,good stability,and specificity in the digital PCR detection system.The establishment of detection methods not only provides technical support for biosafety supervi-sion,but also lays the foundation for the formulation of relevant detection standards.关键词
大豆/基因编辑/油酸/荧光PCR/数字PCRKey words
soybean/gene editing/oleic/fluorescent PCR/digital PCR分类
农业科技引用本文复制引用
袁小雅,甄珍..CRISPR/Cas9基因编辑高油酸大豆快速检测方法研究[J].生物技术进展,2026,16(4):878-884,7.基金项目
海关总署科研项目(2022HK031). （2022HK031）
