生物技术进展2026，Vol.16Issue(4)：898-908,11.DOI:10.19586/j.2095-2341.2025.0148
基于生物纳米孔的第四代测序研究
Research on Fourth-generation Sequencing Based on Biological Nanopores
摘要
Abstract
As a crucial pillar of molecular biology,sequencing technology is experiencing rapid development worldwide.Nano-pore sequencing technology has emerged as the leading fourth-generation sequencing technology,addressing the limitations of previous-generation technologies in terms of throughput,read length,portability and cost.By comparing the key parameters of traditional sequencing and nanopore sequencing,this study elaborated on the principles of biological nanopore sequencing and the composition of sequencing instruments,analyzed the structural characteristics and functional adaptability of biological nano-pore proteins,expounded on the application scenarios of nanopore sequencing in genomics,transcriptomics,epigenetics and oth-er fields,and prospected for the development of sequencing technology.This paper aims to provide a comprehensive reference for researchers to deeply understand nanopore sequencing technology,and facilitates the further promotion,optimization and up-grading of this technology in life science research and clinical practice.关键词
纳米孔测序原理/纳米孔蛋白/基因组学/转录组学/表观遗传学Key words
nanopore sequencing principle/nanopore protein/genomics/transcriptomics/epigenetics分类
生物科学引用本文复制引用
张欣欣,郭甜利,管桦..基于生物纳米孔的第四代测序研究[J].生物技术进展,2026,16(4):898-908,11.基金项目
中央级公益性科研院所基本科研业务费专项资金项目(B25601). （B25601）
