生物技术进展2026，Vol.16Issue(4)：968-978,11.DOI:10.19586/j.2095-2341.2026.0074
高寒矿山土壤解磷菌分离及其固定镉效应研究
Isolation of Phosphate-solubilizing Bacteria from Alpine Mining Soil and Evaluation of Their Cadmium Immobilization Capacity
摘要
Abstract
Phosphate-solubilizing bacteria-mediated biomineralization has great potential for heavy metal remediation,yet its ap-plication efficiency and underlying mechanisms in fragile alpine ecosystems remain poorly understood.A cold-tolerant phosphate-solubilizing strain Serratia quinivorans SH was isolated from an alpine tailings reservoir,and its phosphorus-solubilizing capaci-ty,cadmium(Cd)tolerance and Cd immobilization mechanism under low-temperature conditions were systematically explored.The results showed that the maximum net soluble phosphorus concentration of strain SH reached 210.6 mg·L-1 under low tempera-ture,and the strain exhibited prominent tolerance to Cd stress at 5～40 mg·L-1.Furthermore,the net soluble phosphorus concen-tration ranged from 187.2 to 210.6 mg at 5～15℃,with the optimal pH of 7～8 and optimal C/N ratio of 20∶1.Adsorption-precipi-tation experiments revealed that the maximum net Cd adsorption rate and net precipitation rate of strain SH were 83.3%and 35%,respectively.Characterizations via scanning electron microscopy-energy dispersive X-ray spectroscopy(SEM-EDS)veri-fied that strain SH transformed Cd into stable phosphate precipitates.Whole-genome sequencing analysis further identified func-tional genes involved in phosphorus solubilization and heavy metal resistance.The results showed that the cold-resistant phosphate-solubilizing strain Serratia quinivorans SH possesses low-temperature phosphorus-solubilizing ability and Cd biomineralization ca-pacity,which can provide strain resources and theoretical support for the remediation of heavy metal pollution in alpine tailings.关键词
解磷菌/高寒尾矿/镉污染/生物矿化Key words
phosphate-solubilizing bacteria/alpine tailings/cadmium contamination/biomineralization分类
生物科学引用本文复制引用
韩明贞,刘青海,吴斌,胡俊淇,邱城,潘崇双..高寒矿山土壤解磷菌分离及其固定镉效应研究[J].生物技术进展,2026,16(4):968-978,11.基金项目
西藏自治区中央引导地方项目(XZ202401YD0001) （XZ202401YD0001）
四川省重点研发项目(2025YFNZH0039) （2025YFNZH0039）
成都市科技局项目(2024-YF09-00042-SN). （2024-YF09-00042-SN）
