西北农林科技大学学报（自然科学版）2026，Vol.54Issue(9)：59-66,77,9.DOI:10.13207/j.jnwafu.2026.09.006
柠檬桉种源遗传背景的近红外光谱分析
Near infrared spectroscopy analysis of the genetic background of Corymbia citriodora provenances
摘要
Abstract
[Objective]This study aims to analyze the genetic relationships among different provenances of Corymbia citriodora using near-infrared(NIR)spectral data,and to explore the feasibility and accuracy of ap-plying NIR for genetic analysis.[Method]The study utilized genetic materials from 10 provenances of C.citrio-dora preserved in the National Eucalypt Germplasm Repository.Based on the principles of representativeness and broad sampling,leaf samples from 296 families were collected.For each family,6-10 healthy leaves with consistent physiological conditions were carefully selected,dried,ground,and packed into plastic self sealing bags.NIRs of the powdered samples were acquired using a portable NIR spectrometer(Phazir Rx).After pre-treating the original spectra with Savitzky-Golay second derivative smoothing,a principal component analysis(PCA)model was established.Spectral distances between each pair of the 10 provenances were estimated using Soft Independent Modeling of Class Analogy(SIMCA),an unsupervised discriminant analysis method.A hier-archical clustering tree of provenances was then constructed based on the NIRs distances.Furthermore,the analysis of modeling power at different wavelengths were conducted to elucidate the chemical basis of genetic variations among provenances.[Result]NIRs proved effective for quantifying and distinguishing C.citriodora provenances.The Euclidean spectral distances among provenances ranged from 0.016 to 0.024,clearly cluste-ring the ten provenances into two main groups:native Australian and domestic introduced provenances.The clus-tering pattern of native provenances strongly aligned with their geographic distribution,providing direct evidence for tracing the origins of the introduced germplasms in China.SIMCA analysis further confirmed that the 296 family samples could still be accurately and distinctly classified by their respective models,even for closely re-lated provenances with similar spectral distances.Modeling power analysis highlighted three spectral ranges with particularly high discriminatory power:1 708-1 785,18 80-1 935,and 2 292-2 370 nm.And these waves cor-responded to the characteristic absorption peaks of key chemical compounds and functional groups,namely cellu-lose,hemicellulose,and C―H groups,respectively.The differences in these compounds or functional groups among provenances were likely be the inner reasons of genetic differentiation on C.citriodora.[Conclusion]The present study showed that NIR can effectively reveal the genetic relationships and geographic differentiation among provenances of C.citriodora,which provided a novel solution for clarifying genetic origins of early-intro-duced germplasm of this species in China.关键词
柠檬桉/遗传背景/近红外光谱(NIRs)/光谱距离/层级聚类/判别分析Key words
Corymbia citriodora Hook./genetic background/near-infrared spectroscopy(NIRs)/spectral distance/hierarchical clustering/discriminant analysis分类
农业科技引用本文复制引用
林彦,卢万鸿,翟江博,刘果,罗建中,张鹏..柠檬桉种源遗传背景的近红外光谱分析[J].西北农林科技大学学报（自然科学版）,2026,54(9):59-66,77,9.基金项目
广东省林业科技创新项目(2023KJCX012) （2023KJCX012）
国家重点研发计划子课题(2022YFD2200203-3) （2022YFD2200203-3）
广东省自然科学基金项目(202-1A1515010618) （202-1A1515010618）
