西北农林科技大学学报（自然科学版）2026，Vol.54Issue(9)：171-180,10.DOI:10.13207/j.jnwafu.2026.09.017
万寿菊轮作对半夏连作障碍的改善作用
Improvement effect of Tagetes erecta rotation on continuous cropping obstacle of Pinellia ternata
摘要
Abstract
[Objective]This study aimed to investigate the improvement effect of Tagetes erecta rotation on continuous cropping obstacle of Pinellia ternata,to provide a scientific basis for optimizing P.ternata cultivation and alleviating continuous cropping obstacles.[Method]Two treatments were designed:continuous cropping of Pinellia ternata,and rotation of Pinellia ternata with Tagetes erecta.Conventional methods were employed to determine the growth physiological indicators,soil nutrients content,and soil enzyme activities of P.ternata un-der different treatments.Illumina high-throughput sequencing technology was used to determine the community structure and diversity of soil microorganism.Furthermore,correlation analysis was conducted between rhizo-sphere soil environmental factors and microbial communities under different species patterns of P.ternata.[Re-sult]①Compared with the continuous cropping treatment of P.ternata,the crop rotation with T.erecta signifi-cantly promoted the growth and physiology of P.ternata.The emergence rate,leaf area,plant height,and yield increased significantly by 91.87％,41.30％,29.87％,and 101.51％,respectively(P<0.05).The leaf net pho-tosynthetic rate,stomatal conductance,intercellular CO2 concentration,transpiration rate,and SPAD increased significantly by 20.12％,100.00％,19.96％,48.77％,and 18.35％,respectively(P<0.05).② Compared with the continuous cropping treatment with P.ternata,the T.erecta rotation treatment significantly increased soil pH,nutrient content,and enzyme activities in the rhizosphere soil of P.ternata.Specifically,pH and the con-tents of total nitrogen,total potassium,organic matter increased significantly by 6.60％,26.96％,6.35％,and 6.54％,respectively(P<0.05).Similarly,the activities of soil urease,soil sucrase,and soil polyphenol oxidase increased by 80.22％,14.73％,and 64.79％,respectively(P<0.05).③Compared with continuous cropping of P.ternata,crop rotation with T.erecta restructured the rhizosphere microbial community.Specifically,the di-versity and richness of bacteria increased significantly,whereas no significant differences were observed in fungi.At the phylum level,the relative abundances of Actinomycetota,Acidobacteriota,and Basidiomycota increased by 10.47％,33.37％,and 116.41％,respectively,whereas the relative abundances of Pseudomonadota and As-comycota decreased by 6.48％and 1.38％,respectively.At the genus level,the relative abundance of the genus Arthrobacter increased by 22.20％,while the relative abundances of the genera Nocardioides and Sphingomo-nas decreased by 13.22％and 11.08％,respectively.④Correlation analysis revealed significant positive correla-tions of Bacillota and Cyanobacteria with total soil phosphorus content,and of Myxococcota with soil urease.As-comycota and Zoopagomycota were significantly positively correlated with soil pH and soil urease activity,and significantly negatively correlated with total soil nitrogen content.[Conclusion]T.erecta rotation alleviates the continuous cropping obstacles of P.ternata by increasing soil nutrient content,enhancing soil enzyme activities,and optimizing the rhizosphere microbial community structure,thereby improving soil ecological function and ul-timately promoting plant growth and yield.关键词
半夏栽培/万寿菊/连作障碍/轮作模式/微生物群落结构Key words
Pinellia ternata cultivation/Tagetes erecta/continuous cropping obstacles/crop rotation pattern/microbial community structure分类
农业科技引用本文复制引用
武宝琴,郭清毅,黄浩翔,寇明红,苏雨,万阳,张延红..万寿菊轮作对半夏连作障碍的改善作用[J].西北农林科技大学学报（自然科学版）,2026,54(9):171-180,10.基金项目
甘肃省重点研发计划项目(25YFFA045) （25YFFA045）
