中国草地学报2026，Vol.48Issue(8)：28-41,14.DOI:10.16742/j.zgcdxb.20260020
三种胁迫下紫花苜蓿萌发期耐盐碱性评价及其生理响应规律
Evaluation of Salt-Alkali Tolerance and Physiological Response Patterns of Alfalfa at the Germination Stage under Three Stress Conditions
摘要
Abstract
This study aimed to systematically evaluate the salt-alkali tolerance of alfalfa(Medicago sativa L.)at the germination stage under complex saline-alkali environments and to screen germplasm resources with broad adaptability and stress resistance.Twenty-six alfalfa accessions from different sources were selected and subjected to three stress treatments determined by concentration-gradient screening:200 mmol/L NaCl(salt stress),60 mmol/L NaHCO3(alkali stress),and a combined stress of 50 mmol/L NaCl+50 mmol/L NaHCO3.Eight germination indices,including germination potential,germination percentage,root length,and vigor index,were measured.The membership function method and cluster analysis were used to comprehen-sively evaluate the salt-alkali tolerance of the tested accessions,and representative accessions were further vali-dated using physiological indices.The results showed that different stress types exerted distinctly different inhibitory effects on alfalfa at the germination stage.To obtain an equivalent inhibitory effect,alkali stress required a far lower molar concentration than salt stress,with stronger damaging effects per unit concentration.Cluster analysis revealed significant differences in salt-alkali tolerance among the tested accessions under the three stresses.Although 'WL3251HQ' performed best under single salt stress,it was extremely sensitive to alkali stress.'Felicia' and 'Aurora' ranked among the top in D values under all three stress environments,dem-onstrating excellent and stable comprehensive salt-alkali tolerance,and were classified as strongly salt-alkali-tolerant accessions.'Dripperstar(Multi-Leaf)' and 'WL366HQ' were identified as salt-alkali-sensitive acces-sions.Physiological verification showed that the strongly salt-alkali-tolerant accession Aurora could maintain high antioxidant enzyme activities under stress and effectively alleviate membrane lipid peroxidation,and its physiological response patterns were consistent with the results of the comprehensive evaluation.This study screened accessions with stable performance under multiple salt-alkali stresses,thereby providing a theoretical basis and material foundation for alfalfa breeding and cultivation in salt-alkali land.关键词
紫花苜蓿/盐碱胁迫/种子萌发/隶属函数法/评价指标Key words
Alfalfa/Salt-alkali stress/Seed germination/Membership function method/Evaluation indicators分类
农业科技引用本文复制引用
王浩杰,卜令贝,李姣,黄炎,李园园,田思瑶,于辉,朱昊,张延辉..三种胁迫下紫花苜蓿萌发期耐盐碱性评价及其生理响应规律[J].中国草地学报,2026,48(8):28-41,14.基金项目
塔里木河流域巴州北部沙漠盐渍化与生态恢复调查监测与评价项目(WZD-YW-2025-203) （WZD-YW-2025-203）
