中国草地学报2026，Vol.48Issue(8)：73-81,9.DOI:10.16742/j.zgcdxb.20250419
纤维素酶添加剂对饿蚂蝗干草营养品质及真菌群落的影响
Effects of Cellulase Additive on Nutritional Quality and Fungal Community of Desmodium multiflorum Hay
摘要
Abstract
To investigate the effects of different cellulase additive levels on the nutritional quality of Desmo-dium multiflorum hay and the fungal community after 30 d of storage,the aboveground tender stems and leaves of D.multiflorum were used as materials,and five cellulase addition levels(0,25,50,75,and 100 mg/kg)were set up.Nutrient composition was determined at 0,30,and 60 d of storage,and fungal community analy-sis was performed on samples stored for 30 d.The results showed that cellulase addition had extremely signifi-cant effects on the contents of soluble sugar,crude protein,ether extract,and crude ash(P<0.01),a signifi-cant effect on neutral detergent fiber content(P<0.05),and no significant effect on acid detergent fiber and tan-nin contents.Overall,cellulase addition increased crude protein content and decreased neutral detergent fiber content,whereas soluble sugar,ether extract,and crude ash contents fluctuated to varying degrees among cellu-lase addition levels.Storage time had extremely significant effects on ether extract,crude ash,and tannin con-tents(P<0.01),and significant effects on neutral detergent fiber and acid detergent fiber contents(P<0.05).With prolonged storage,ether extract content decreased,while crude ash,neutral detergent fiber,acid deter-gent fiber,and tannin contents increased.After 30 d of storage,Ascomycota and Basidiomycota were the domi-nant fungal phyla in all treatments,and the predominant genera included Cladosporium,Cercospora,Remoti-didymella,Vishniacozyma,Setophoma,and Fusarium,with certain differences in relative abundance among cellulase addition levels.The fungal species richness in the 75 mg/kg treatment was significantly lower than that in the control(P<0.05),while the other α-diversity indices showed no significant differences from the con-trol.Grey relational analysis based on nutrient composition and fungal community characteristics showed that the 25 mg/kg cellulase treatment had the highest equal-weight relational degree,indicating a relatively superior com-prehensive evaluation.关键词
饿蚂蝗/纤维素酶/干草品质/营养成分/真菌群落Key words
Desmodium multiflorum/Cellulase/Hay quality/Nutrient composition/Fungal community分类
农业科技引用本文复制引用
张一桦,王从文,沙曜,李屏贤,尹以昌,何绍笛,段新慧,赵志丽..纤维素酶添加剂对饿蚂蝗干草营养品质及真菌群落的影响[J].中国草地学报,2026,48(8):73-81,9.基金项目
云南省重大科技专项计划(202502AE090060) （202502AE090060）
云南省乡村振兴专项计划(202404BU090032) （202404BU090032）
