中国肿瘤生物治疗杂志2026，Vol.33Issue(8)：857-866,10.DOI:10.3872/j.issn.1007-385X.2026.08.006
miR-532-3p靶向DDOST抑制甲状腺癌TPC-1细胞增殖、糖酵解及裸小鼠移植瘤生长
miR-532-3p targets DDOST and inhibits thyroid cancer TPC-1 cell proliferation,glycolysis,and xenograft tumor growth in nude mice
徐慧海 1王文帅 1王倩 1杜秀然 1李姿健2
作者信息
- 1. 河北省胸科医院 普通外科,河北 石家庄 050000
- 2. 河北省胸科医院 胸外科,河北 石家庄 050000
- 折叠
摘要
Abstract
Objective:This study aimed to investigate the effects of miR-532-3p on thyroid cancer TPC-1 cell proliferation and glycolysis and to preliminarily explore the underlying mechanism by targeting dolichyl-diphosphooligosaccharide protein glycosyltransferase non-catalytic subunit(DDOST).Methods:The basal expression of miR-532-3p was assessed in normal thyroid Nthy-ori 3-1 cells and thyroid cancer cell lines TPC-1,FTC-133,and IHH-4.TPC-1 cells were selected for subsequent experiments and divided into mimic-NC group,miR-532-3p mimic group,inhibitor-NC group,miR-532-3p inhibitor group,mimic-NC+pcDNA-NC group,mimic-NC+pcDNA-DDOST group,miR-532-3p mimic+pcDNA-NC group,and miR-532-3p mimic+pcDNA-DDOST group.The targeting relationship between miR-532-3p and DDOST was detected by dual-luciferase reporter gene assay.Cell proliferation was detected by the CCK-8 method.Gene and protein expression levels were detected by reverse transcription quantitative PCR(RT-qPCR)and Western blotting.The TPC-1 cells were subcutaneously injected into the right posterior axillary region of nude mice to establish a xenograft tumor model of thyroid carcinoma,and the growth status of the tumors was recorded.Ki-67 and DDOST expression in xenograft tumor tissues was detected by immunohistochemistry.Lactate concentration and glucose consumption were measured using the corresponding assay kits.Results:Compared with normal thyroid cells Nthy-ori 3-1,the expression levels of miR-532-3p in thyroid cancer cell lines(TPC-1,FTC-133,IHH-4)were significantly decreased(P<0.05),with the lowest level observed in TPC-1 cells.The expression of DDOST was significantly increased(P<0.05).Overexpression of miR-532-3p inhibited TPC-1 cell proliferation and glycolysis and downregulated DDOST expression(P<0.05).Moreover,these effects were reversed by DDOST overexpression(P<0.05).Overexpression of miR-532-3p inhibited thyroid cancer xenograft growth and reduced DDOST expression in xenograft tumor tissues(P<0.05).Conclusion:Overexpression of miR-532-3p inhibited TPC-1 cell proliferation and glycolysis by targeting DDOST and suppressed thyroid cancer xenograft growth in nude mice.关键词
miR-532-3p/多萜醇二磷酸寡糖蛋白糖基转移 酶非催化亚基/增殖/糖酵解/甲状腺癌Key words
miR-532-3p/DDOST/proliferation/glycolysis/thyroid cancer分类
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徐慧海,王文帅,王倩,杜秀然,李姿健..miR-532-3p靶向DDOST抑制甲状腺癌TPC-1细胞增殖、糖酵解及裸小鼠移植瘤生长[J].中国肿瘤生物治疗杂志,2026,33(8):857-866,10.
