中国中医基础医学杂志2026，Vol.32Issue(9)：1939-1946,8.
健脾补肾方调控p53/p21细胞衰老通路抑制辐射小鼠造血系统损伤的机制研究
Mechanism of Jianpi Bushen Prescription in Inhibiting Hematopoietic System Damage via the p53/p21 Cell Senescence Pathway in Radiation-Exposed Mice
摘要
Abstract
Objective To observe the regulatory effect of Jianpi Bushen prescription on the p53/p21 cell senescence signaling pathway in a radiation-induced mouse model,and to explore its mechanism in inhibiting hematopoietic system damage.Methods Among 30 mice,6 were randomly selected as the normal control group,and the remaining 24 mice received a single whole-body irradiation of 6.0 Gy X-ray to establish the model.After modeling,the mice were randomly divided into model group,low-dose Jianpi Bushen prescription group,high-dose Jianpi Bushen prescription group,and compound E-jiao slurry group.On day 14 after intragastric administration,peripheral blood was collected,femurs were isolated,and bone marrow was harvested.White blood cell(WBC)count,hemoglobin(Hb),and platelet(PLT)levels were measured using a hematology analyzer.Bone marrow histopathological changes were observed by hematoxylin-eosin(HE)staining.Senescence-associated β-galactosidase(SA-β-Gal)Concentration in bone marrow was detected by ELISA.Bone marrow cell cycle was analyzed by flow cytometry.The mRNA expression levels of p53,p21,CyclinE1,and CDK2 in bone marrow tissue were detected by RT-qPCR.The protein expression levels of p53,p-p53,p21,CyclinE1,and CDK2 were detected by Western blot.Results Compared with the normal group,the model group showed significantly decreased WBC count,Hb,and PLT levels(P<0.01),marked destruction of bone marrow tissue structure,extensive necrosis of bone marrow cells,significantly reduced bone marrow cell count,significantly increased adipocytes,significantly elevated SA-β-Gal Concentration(P<0.01),significantly increased proportion of cells in G0/G1 phase(P<0.01),significantly decreased proportions of cells in S and G2/M phases(P<0.01),significantly upregulated mRNA and protein expression of p53 and p21(P<0.01),and significantly downregulated mRNA and protein expression of CyclinE1 and CDK2(P<0.01).Compared with the model group,all drug intervention groups significantly improved the above indicators(P<0.05).Among the drug intervention groups,the low-dose Jianpi Bushen prescription group showed better efficacy than the high-dose group(P<0.05),and no significant difference was observed compared with the compound E-jiao slurry group(P>0.05).Conclusion Jianpi Bushen prescription may promote the recovery of hematopoietic system damage by inhibiting the p53/p21 cell senescence pathway,thereby improving the senescence state of hematopoietic cells,promoting hematopoietic cell proliferation,and increasing peripheral blood cell counts.关键词
健脾补肾方/p53/p21/细胞衰老/辐射损伤Key words
Jianpi Bushen prescription/p53/p21/Cell senescence/Radiation injury分类
医药卫生引用本文复制引用
肖静静,何东初,张梦云,张勇,丁晓娟,伍伟..健脾补肾方调控p53/p21细胞衰老通路抑制辐射小鼠造血系统损伤的机制研究[J].中国中医基础医学杂志,2026,32(9):1939-1946,8.基金项目
国家自然科学基金面上项目(82574833,81873153,81273905) （82574833,81873153,81273905）
湖北省中医药管理局中医药重点项目(ZY2023Z010) （ZY2023Z010）
湖北省自然科学基金青年项目(2024AFB538) （2024AFB538）
