中南民族大学学报（自然科学版）2026，Vol.45Issue(6)：775-787,13.DOI:10.20056/j.cnki.ZNMDZK.20260754
网络药理学及单细胞测序技术对山竹醇抗乳腺癌潜在通路及上皮细胞亚群关联分析
Association analysis of core pathways and epithelial cell subsets in garcinol-mediated anti-breast cancer using network pharmacology combined with single-cell RNA sequencing
摘要
Abstract
To investigate the mechanism of action of garcinol against breast cancer and the characteristics of targeted association with epithelial cell subsets,283 potential targets of garcinol and 4560 targets related to breast cancer were screened by network pharmacology,192 intersection targets were obtained,PPI network was constructed and core targets such as TP53,SRC,and AKT1 were selected;GO/KEGG enrichment analysis showed that potential targets were mainly concentrated in tumor-related pathways such as pathway signaling in cancer and MAPK pathway.Molecular docking verified that garcinol had good binding activity(binding energy≤-5.0 kcal/mol)to potential targets,with binding energy-8.7 kcal/mol to STAT3.Cell experiments showed that garcinol concentration-dependently inhibited the proliferation of two breast cancer cells and down-regulated mRNA transcription and post-translational modifications(p-STAT3,p-AKT and p-SRC).Single-cell sequencing analysis identified the subset composition of MCF-7 and MDA-MB-231 cells and selected key epithelial cell subsets whose potential targeted pathways closely matched those predicted by network pharmacology.Intersection analysis revealed potential genes and pathways involved in the regulation of malignant phenotypes of MCF-7(proliferation-apoptosis)and MDA-MB-231(invasion-migration)by garcinol.The study reveals the specific regulatory mechanism of garcinol against breast cancer and provides a theoretical basis for individualized treatment of breast cancer and clinical translation of garcinol.关键词
山竹醇/乳腺癌/网络药理学/单细胞测序Key words
garcinol/breast cancer/network pharmacology/single-cell sequencing分类
医药卫生引用本文复制引用
张文龙,刘金虹,姚慧,胡忆榕,胡鑫..网络药理学及单细胞测序技术对山竹醇抗乳腺癌潜在通路及上皮细胞亚群关联分析[J].中南民族大学学报（自然科学版）,2026,45(6):775-787,13.基金项目
国家自然科学基金资助项目(81201610) （81201610）
湖北省自然科学基金资助项目(2023AFB681) （2023AFB681）
中央高校基本科研业务费专项资金资助项目(CZY17014) （CZY17014）
