植物营养与肥料学报2026，Vol.32Issue(8)：1635-1644,10.DOI:10.11674/zwyf.2025502
生物和化学硝化抑制剂在红壤上的硝化抑制效果及驱动因素
Nitrification inhibition effect of biological and chemical nitrification inhibitors in red soils and the influencing factors
摘要
Abstract
[Objectives]The application of nitrification inhibitors(NIs)is an effective measure to suppress soil nitrification and reduce nitrogen losses in agricultural ecosystems.However,the efficacy of NIs is influenced by both types of NIs and fertilization management practices.Further investigation is urgently required to elucidate the inhibitory effects of NIs on soil nitrification and underlying mechanisms.[Methods]An incubation experiment was conducted using red soils collected from a long-term fertilization field experiment in Jinxian,Jiangxi Province,China.The soils were derived from four treatments:unfertilized control(Control),mineral fertilizer(NPK),organic fertilizer(OM),and combined mineral and organic fertilizer(NPKM).Three nitrification inhibitor treatments were established for each soil:no nitrification inhibitor(NoNI),the biological nitrification inhibitor methyl 3-(4-hydroxyphenyl)propionate(MHPP),and the chemical nitrification inhibitor 3,4-dimethylpyrazole phosphate(DMPP).Soil physicochemical properties were determined before incubation,and soil samples were collected on days 0,1,3,and 6 to measure NH4+-N and NO3--N contents.Net nitrification rate,nitrification inhibition rates,and their relationships with soil properties were analyzed.[Results]Compared with NoNI,MHPP treatment reduced the net nitrification rates of the Control,OM,and NPKM treatment soils by 62.04％,59.57％,and 39.73％,respectively,with nitrification inhibition rates remaining at approximately 40％or higher and being higher than those of DMPP in the OM and NPKM treatments;DMPP treatment reduced the net nitrification rates by approximately 60％and 40％in the OM and NPKM treatments,with inhibition rates of about 60％and 40％,respectively,but showed no significant inhibitory effect in the Control and NPK treatments.In the OM and NPKM treatments,both inhibitors delayed NH4+-N transformation and reduced NO3--N accumulation,although their inhibitory effects were significantly regulated by fertilization regimes.Correlation analysis indicated that the inhibitory effect of MHPP was mainly associated with soil C:N and pH,whereas the effectiveness of DMPP was more strongly related to soil pH and organic carbon content.[Conclusions]Long-term fertilization alters soil pH,organic carbon content,and C:N ratio,thereby affecting the performance of nitrification inhibitors in red soils.Under organic fertilizer and combined organic-mineral fertilizer systems,both MHPP and DMPP effectively suppressed nitrification,with MHPP showing more stable inhibitory performance.In contrast,DMPP is more suitable than MHPP in soils receiving only mineral fertilizer.关键词
红壤/生物硝化抑制剂/化学硝化抑制剂/净硝化速率/硝化抑制率/土壤理化性状Key words
red soil/biological nitrification inhibitor/chemical nitrification inhibitor/net nitrification rate/nitrification inhibition rate/soil physicochemical property引用本文复制引用
李尔,陈美淇,柳开楼,陈金,程谊..生物和化学硝化抑制剂在红壤上的硝化抑制效果及驱动因素[J].植物营养与肥料学报,2026,32(8):1635-1644,10.基金项目
国家自然科学基金项目(42425705,42407460) （42425705,42407460）
江西省引进培养创新创业高层次人才"千人计划"(S2021DQKJ0001). （S2021DQKJ0001）
