中医药信息2026，Vol.43Issue(9)：1-10,10.DOI:10.19656/j.cnki.1002-2406.20260901
基于转录组和蛋白质组学及实验验证探究通脑饮减轻大鼠脑缺血再灌注损伤的机制
Investigation on Mechanism of Tongnao Decoction in Alleviating Cerebral Ischemia-Reperfusion Injury in Rats Based on Transcriptomics,Proteomics and Experimental Verification
摘要
Abstract
Objective To investigate the molecular targets and mechanism of Tongnao Decoction(TND)in alleviating cerebral ischemia-reperfusion injury(CIRI).Methods A total of eighteen Sprague-Dawley(SD)rats were randomly divided into a control group(sham operation group),a model group and a TND group,with 6 rats in each group.The middle cerebral artery occlusion(MCAO)model was used to simulate CIRI in the model group and the TND group.After the successful modeling,the TND group received water extract of TND by daily intragastric administration at a dose of 10 g/(kg·d),while the control group and the model group were gavaged with sterile distilled water.All groups received the administration volume at 1 mL/100 g body weight for 1 week.Neurological deficit severity was evaluated by Zea Longa score;cerebral infarct volume was observed by 2,3,5-triphenyltetrazolium chloride(TTC)staining;histopathological morphology of brain tissue was observed by hematoxylin-eosin(HE)staining to assess the neuroprotective effect.Transcriptomics and proteomics were combined to screen differentially expressed genes/proteins and overlapping targets between control group vs.model group and model group vs.TND group,followed by functional enrichment analysis.Finally,key targets were verified by qRT-PCR and Western blot.Results Compared with the control group,the model group showed a significantly higher Zea Longa score(P<0.01),and a larger percentage of infarct area(P<0.01).Compared with the model group,the Zea Longa score was significantly decreased(P<0.01)and a smaller percentage of infarct area was observed(P<0.01)in the TND group.Integrated transcriptomic and proteomic analysis revealed that TND significantly modulated the complement and coagulation cascades pathway and the ECM-receptor interaction pathway.Molecular biological experiments confirmed that TND upregulated the genes of SPP1,Fga,Serpinf2,F2,A2m,Itga5,Kng2,C4b,and Usp18,as well as the protein expressions of SPP1 and Serpinf2.Its mechanism may be related to the regulation of complement and coagulation cascade and ECM-receptor interaction pathway.Conclusion Tongnao Decoction may exert alleviation effect on CIRI by regulating complement and coagulation cascade and ECM-receptor interaction.关键词
通脑饮/脑缺血再灌注损伤/转录组学/蛋白质组学/补体/凝血级联/ECM-受体相互作用Key words
Tongnao Decoction/Cerebral ischemia-reperfusion injury/Transcriptomics/Proteomics/Complement and coagulation cascade/ECM-receptor interaction引用本文复制引用
杜琳琳,陈兆耀,宋繁涛,厉励,贺秋画,张秋池,沈佳心,姚蓓蓓,吴明华,周源..基于转录组和蛋白质组学及实验验证探究通脑饮减轻大鼠脑缺血再灌注损伤的机制[J].中医药信息,2026,43(9):1-10,10.基金项目
国家自然科学基金项目(82274428) （82274428）
江苏省卫健委医学科研重点项目(K2023009) （K2023009）
南京中医药大学自然科学基金面上项目(XZR2023030) （XZR2023030）
江苏省中医院创新发展基金项目(Y22011) （Y22011）
国家科技重大专项"癌症、心脑血管、呼吸和代谢性疾病防治研究"项目(2024ZD0522103) （2024ZD0522103）
