生物资源2026，Vol.48Issue(4)：352-360,9.DOI:10.14188/j.ajsh.20260036
深海高抗菌活性真菌的分离筛选及优势菌株的初步鉴定
Isolation and screening of deep-sea fungi with high antimicrobial activity and its dominant strain preliminary identification
摘要
Abstract
Microbial drug resistance poses a critical global public health challenge,and fungi in extreme habitats are core potential resources for exploring new antimicrobial lead compounds.This study aimed to establish and validate a three-level stepwise screening strategy including morphological de-replication,dual-method antimicrobial activity re-screening,and HPLC chemical diversity assessment,and to apply it to the systematic exploration of deep-sea culturable fungi.A total of 138 independent pure culture strains were isolated from 30 deep-sea sediment samples using gradient dilution plating combined with four selective media.Crude extracts were prepared via dual solid-liquid fermentation.Antimicrobial activity evaluation of crude extracts was completed through preliminary screening by plate punch method and re-screening by 96-well plate-microplate reader.Further HPLC chemical diversity analysis was used to screen the candidate strain.The candidate strain WHU10116 was selected and subjected to morphological microscopy and ITS rDNA phylogenetic identification.Results showed that the positive rates of antimicrobial activity of 138 strains against Escherichia coli,Staphylococcus aureus,and Candida albicans were 96.4%,58.0%,and 16.7%,respectively.ITS rDNA phylogenetic analysis(maximum likelihood)placed strain WHU10116 within Aspergillus section Aspergillus,forming a clade with A.chevalieri,A.intermedius,and A.costiformis(bootstrap=96%),within which WHU10116 is most closely related to A.chevalieri;however,its species-level assignment still requires multi-gene phylogenetic analysis.The strain WHU10116,with a unique habitat origin,broad-spectrum antimicrobial activity,and abundant metabolic characteristics,is a priority candidate for subsequent metabolite isolation,identification,and genome mining.This study provides a methodological reference for the pharmaceutical exploration of deep-sea culturable fungi.关键词
真菌分离/抗菌活性/曲霉属曲霉组/内转录间隔区核糖体DNA/代谢产物多样性/高效液相色谱Key words
fungal isolation/antimicrobial activity/Aspergillus section Aspergillus/ITS rDNA/metabolite diversity/HPLC分类
生物科学引用本文复制引用
刘群娣,陶韦新,朱冬青,洪葵..深海高抗菌活性真菌的分离筛选及优势菌株的初步鉴定[J].生物资源,2026,48(4):352-360,9.基金项目
国家重点研发计划(2024YFC2815900) （2024YFC2815900）
