生物资源2026，Vol.48Issue(4)：372-380,9.DOI:10.14188/j.ajsh.20260302001
黄孢原毛平革菌BZ103液体与固体发酵降解小麦秸秆效能比较
Comparison of wheat straw degradation by Phanerochaete chrysosporium BZ103 under submerged and solid-state fermentation
摘要
Abstract
The green bioconversion of agricultural straw is of great significance for reducing environmental pollution and promoting the resource utilization of agricultural waste.In this study,Phanerochaete chrysosporium BZ103 was used to compare enzyme expression,lignocellulosic component degradation,and structural changes in wheat straw under submerged fermentation(SmF)and solid-state fermentation(SSF).The results showed that SmF was more favorable for the expression of lignin-degrading enzymes,with the maximum activities of manganese peroxidase and lignin peroxidase reaching 1 415 U/g and 1 784 U/g,respectively.After 12 d of fermentation,the lignin degradation rate under SmF reached 40.34%,which was significantly higher than that under SSF(34.14%).In contrast,SSF was more favorable for the expression of some cellulolytic enzymes during the early and middle stages of fermentation,with the maximum activities of endoglucanase and β-glucosidase reaching 4.8 U/g and 9.1 U/g,respectively,both higher than those under SmF.Scanning electron microscopy(SEM),attenuated total reflectance Fourier transform infrared spectroscopy(ATR-FTIR),and X-ray diffraction(XRD)analysis showed that SmF caused faster disruption of the straw surface structure and more pronounced attenuation of lignin-related characteristic functional groups,whereas SSF exerted a stronger effect on cellulose-related structural features and crystalline regions,with the crystallinity index decreasing to 38.07%,lower than that under SmF(40.64%).These results indicate that,under the conditions of this study,SmF shows a more pronounced selective delignification effect,whereas SSF is more favorable for the loosening of cellulose structure.This study provides an experimental basis for selecting suitable biological pretreatment strategies for the wheat straw resource utilization by P.chrysosporium BZ103.关键词
小麦秸秆/黄孢原毛平革菌/选择性脱木质素/木质素降解酶/发酵方式Key words
wheat straw/Phanerochaete chrysosporium/selective delignification/lignin-degrading enzymes/fermentation mode分类
生物科学引用本文复制引用
王馨宜,徐海舟,刘舒欣,陈曦,张殿朋,赵洪新..黄孢原毛平革菌BZ103液体与固体发酵降解小麦秸秆效能比较[J].生物资源,2026,48(4):372-380,9.基金项目
北京市食用菌创新团队项目(BAIC03-19-2026) （BAIC03-19-2026）
浙江省产业技术团队项目(浙农科发[2025]5号) （浙农科发[2025]5号）
