生物资源2026，Vol.48Issue(4)：400-408,9.DOI:10.14188/j.ajsh.20260045
高糖饮食调控秀丽隐杆线虫生物钟的分子机制研究
Molecular mechanism of high-glucose diet regulating the biological clock of Caenorhabditis elegans
摘要
Abstract
Physiological processes and behaviors in almost all organisms exhibit intrinsic rhythmicity.These biological rhythms are generated by a transcriptional-translational negative feedback loop involving multiple biological clock genes.High-carbohydrate diets are known to affect biological rhythms in mammals by modulating the expression of biological clock genes,but the underlying molecular mechanisms remain poorly understood.This study established a fluorescent reporter system for lin-42,the Caenorhabditis elegans homolog of the core clock gene Period.The fluorescence reporter and real-time quantitative PCR results revealed that dietary glucose supplementation significantly altered the expression rhythm of lin-42/Period.In addition to lin-42,the high-glucose diet induced aberrant upregulation of several other biological clock-related genes in C.elegans.Further mechanism studies showed that loss of function of MML-1,the C.elegans homolog for the evolution-conserved glucose-sensing transcription factors MondoA(also known as MLXIP,MLX-interacting protein)and ChREBP(carbohydrate response element-binding protein),effectively suppressed the disruption of lin-42 expression rhythms induced by excess glucose.These findings indicate that rhythmic regulation of C.elegans biological clock gene expression by excessive glucose is dependent on MML-1/MondoA.Collectively,our findings not only demonstrate the conserved role for dietary glucose in the regulation of biological clocks,but also identify a key molecule mediating the coupling between metabolic pathways and the biological clock system.关键词
生物钟/高糖饮食/Period/MondoA/ChREBP/秀丽隐杆线虫Key words
biological clock/high-glucose diet/Period/MondoA/ChREBP/Caenorhabditis elegans分类
生物科学引用本文复制引用
李丽,朱怡,汪琴,张惠敏,傅容..高糖饮食调控秀丽隐杆线虫生物钟的分子机制研究[J].生物资源,2026,48(4):400-408,9.基金项目
国家自然科学基金(32571028) （32571028）
江苏省自然科学基金资助项目(BK20200866) （BK20200866）
江苏省高校重点学科建设资助项目(PAPD) （PAPD）
