波谱学杂志2026，Vol.43Issue(3)：350-366,17.DOI:10.11938/cjmr20253191
脉冲偶极耦合EPR技术在生物大分子结构变化与互作研究中的应用
Applications of Pulsed Dipolar EPR Spectroscopy in Characterizing Interactions and Structural Changes of Biomacromolecules
摘要
Abstract
Pulsed dipolar electron paramagnetic resonance(PD-EPR)is a powerful biophysical technique for probing the dynamic structures and interactions of biomacromolecules such as proteins.By measuring dipolar interactions between unpaired electrons,PD-EPR directly yields nanometer-scale distance distributions between electron spins,from which conformational transitions can be inferred by comparison under different experimental conditions.This review summarizes three major PD-EPR techniques,including double electron-electron resonance,double quantum coherence,and relaxation-induced dipolar modulation enhancement,together with commonly used spin labels and protein spin-labeling strategies,highlighting recent advances in the study of biomacromolecules and complexes.关键词
脉冲偶极耦合EPR/蛋白质动态结构/自旋标记/生物大分子/双电子-电子共振Key words
pulsed dipolar EPR/protein dynamic structures/spin labeling/biomacromolecules/DEER分类
数理科学引用本文复制引用
谢雅欣,杨茵,苏循成..脉冲偶极耦合EPR技术在生物大分子结构变化与互作研究中的应用[J].波谱学杂志,2026,43(3):350-366,17.基金项目
国家自然科学基金资助项目(22177056,22574083) （22177056,22574083）
天津市自然科学基金资助项目(22JCYBJC01490). （22JCYBJC01490）
