草业科学2026，Vol.43Issue(8)：1999-2013,15.DOI:10.11829/j.issn.1001-0629.2024-0808
花苜蓿CIPK基因家族鉴定及干旱胁迫下的表达分析
Genome-wide identification and expression analysis of the CIPK gene family in Medicago ruthenica under drought stress
摘要
Abstract
CIPK(calcineurin B-like interacting protein kinase)is a protein that binds to calcium-ion signal response elements and plays a significant role in plant responses to abiotic stress.In this study,25 CIPK gene family members were identified in the Medicago ruthenica genome through local homology alignment and hidden Markov model searches and were divided into four subfamilies(Group Ⅰ-Group Ⅳ).The remaining members were distributed across seven chromosomes,except for MrCIPK24 and MrCIPK25,which were not assembled on chromosomes.Gene structure and conserved domain analyses indicated that the family members could be divided into two types,intron-rich and intron-lacking,with the majority of the motifs being highly conserved.Expression pattern analysis under drought stress and real-time fluorescent quantitative PCR results showed that expression of the majority of the MrCIPK genes was affected by drought stress,among which the MrCIPK02 gene may have a positive regulatory effect on M.ruthenica's response to drought stress.This study provides theoretical support for subsequent functional studies of the CIPK gene family members in M.ruthenica.关键词
全基因组鉴定/CIPK蛋白激酶/钙信号通路/表达模式分析/抗旱分子机制/实时荧光定量PCR/植物抗逆性Key words
genome-wide identification/CIPK protein kinase/calcium signaling pathway/expression pattern analysis/molecular mechanism of drought resistance/quantitative real-time PCR/plant stress tolerance引用本文复制引用
章磊,严伟,罗栋,王召明,刘志鹏,周强..花苜蓿CIPK基因家族鉴定及干旱胁迫下的表达分析[J].草业科学,2026,43(8):1999-2013,15.基金项目
内蒙古自治区"揭榜挂帅"项目(2022JBGS0040) （2022JBGS0040）
2023年国家草业技术创新中心(筹)重大创新平台建设专项(CCPTZX2023N04) （筹）
甘肃省科技重大专项(23ZDNA009) （23ZDNA009）
