草地学报2026，Vol.34Issue(9)：3164-3171,8.DOI:10.11733/j.issn.1007-0435.2026.09.003
杂交狼尾草重离子辐射诱变体系及耐寒种质筛选
Heavy-Ion Radiation Mutagenesis System and Screening of Cold-Tolerant Germplasm in Hybrid Pennisetum
温昊 1王晓荣 1侯波 1陈曦 1张蕴薇 1倪成成 1毕晓静1
作者信息
- 1. 中国农业大学草业科学与技术学院,北京 100193
- 折叠
摘要
Abstract
Hybrid Pennisetum(Pennisetum glaucum×P.purpureum)is an important high-yield and high-quality forage grass;however,its poor cold tolerance restricts its cultivation and utilization in northern and high-latitude regions.Heavy-ion irradiation is a relatively new mutagenic approach and has been rarely applied in forage breed-ing.In this study,seeds of the hybrid Pennisetum cultivar'Bangde No.1'were treated with heavy-ion irradiation at doses of 50,100,150,200,and 300 Gy.Seedling growth traits and physiological responses under low-tempera-ture stress were systematically evaluated to investigate the mutagenic effects of heavy-ion irradiation,establish a heavy-ion mutagenesis system for hybrid Pennisetum seeds,and develop novel cold-tolerant germplasm.The results showed that heavy-ion irradiation exerted a clear dose-dependent effect on hybrid Pennisetum.Among the treatments,100 Gy not only maintained a high seedling establishment rate but also significantly increased soluble sugar content and the activities of superoxide dismutase(SOD)and peroxidase(POD),reduced membrane damage,and resulted in the lowest semi-lethal temperature(LT50)of plant mutants,indicating the strongest cold tolerance and relatively high mutagenic efficiency.Based on a comprehensive evaluation integrating laboratory and field assessments,principal component analysis,membership function analysis,field survival rate,and field over-wintering rate,three potential cold-tolerant mutants were identified from the M₁ generation.关键词
杂交狼尾草/重离子辐射/诱变育种/耐寒种质/诱变技术体系Key words
Hybrid Pennisetum/Heavy-ion irradiation/Mutation breeding/Cold-tolerant germplasm/Mutagenesis system分类
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温昊,王晓荣,侯波,陈曦,张蕴薇,倪成成,毕晓静..杂交狼尾草重离子辐射诱变体系及耐寒种质筛选[J].草地学报,2026,34(9):3164-3171,8.
