草地学报2026，Vol.34Issue(9)：3197-3210,14.DOI:10.11733/j.issn.1007-0435.2026.09.006
狼尾草WRKY基因家族全基因组鉴定与冷胁迫表达分析
Genome-Wide Identification and Cold Stress Expression Analysis of the WRKY Gene Family in Pennisetum alopecuroides
史志福 1王鑫 2岳跃森 2滕珂 2周仂 2刘凌云 2袁苏凡 2范希峰 2穆娜2
作者信息
- 1. 北京农学院,北京 102206||北京市农林科学院草业花卉与景观生态研究所,北京 100097
- 2. 北京市农林科学院草业花卉与景观生态研究所,北京 100097
- 折叠
摘要
Abstract
To clarify the molecular characteristics of the WRKY gene family in highly cold-resistant Pennisetum alopecuroides and their functions in cold stress response,in this study we conducted systematic identification,phylogenetic analysis and cold stress-related expression pattern analysis of the WRKY gene family via combined genome-wide and transcriptome analyses.The results showed that a total of 104 PaWRKY genes with com-plete WRKY domains were identified,which were unevenly distributed on nine chromosomes.All the encoded proteins were hydrophilic,and most of them had poor in vitro stability and were susceptible to degradation.Based on phylogenetic analysis,the taxa were divided into seven subtribes,and Subtribe IIc comprises the most members,and ten core conserved motifs were predicted in total.Extensive homologous collinearity was found between this gene family and Oryza sativa subsp.japonica.The promoter regions of PaWRKY genes were enriched with numerous cis-acting elements responsive to abiotic stresses and phytohormones.Transcriptome data revealed that eight PaWRKY genes were differentially expressed under cold stress,including four upregu-lated and four downregulated genes,all of which harbored MeJA-responsive elements.This study clarified the molecular and evolutionary characteristics of the WRKY gene family in Pennisetum alopecuroides and screened out key cold stress-responsive genes,which laid a theoretical foundation for cold-tolerant molecular breeding of Pennisetum alopecuroides and evolutionary research of the WRKY gene family in Panicoideae.关键词
狼尾草/WRKY 基因家族/冷胁迫/差异表达Key words
Pennisetum alopecuroides/WRKY gene family/Cold stress/Differential expression分类
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史志福,王鑫,岳跃森,滕珂,周仂,刘凌云,袁苏凡,范希峰,穆娜..狼尾草WRKY基因家族全基因组鉴定与冷胁迫表达分析[J].草地学报,2026,34(9):3197-3210,14.
