草地学报2026，Vol.34Issue(9)：3223-3233,11.DOI:10.11733/j.issn.1007-0435.2026.09.008
象草ERF基因家族鉴定及其对低温胁迫的响应分析
Identification of the ERF Gene Family in Pennisetum Purpureum and Analysis its Response to Low-Temperature Stress
杨玉娇 1贺朝鑫 1周洁 1李林菊 1张新全 1彭燕1
作者信息
- 1. 四川农业大学草业科技学院,四川 成都 611300
- 折叠
摘要
Abstract
Ethylene response factors(ERFs)are key regulatory proteins involved in plant responses to abiotic stresses.In this study,based on the genome data of Pennisetum purpureum,53 members of the PpERF gene family were identified.The encoded proteins ranged from 191 to 1145 amino acids in length,with predicted isoelectric points ranging from 4.75 to 11.79 and molecular weights ranging from 20 536.11 to 124 866.3 Da.Among these genes,three were not mapped to the chromosomes,whereas the remaining genes were unevenly distributed across eight chromosomes.Phylogenetic analysis classified the PpERF genes into three evolutionary clades.Most PpERF proteins contained highly conserved Motifs 1,2,and 4,and multiple stress-responsive cis-acting elements were identified in their promoter regions.Expression analysis showed that 11 PpERF genes exhibited spatiotemporal expression specificity under low-temperature stress,among which PpERF17,PpERF21,and PpERF32 were sig-nificantly upregulated after 24 h of cold treatment.Transient overexpression assays in tobacco further demonstrated that these three genes significantly enhanced plant tolerance to low-temperature stress.This study provides a theo-retical basis for elucidating the molecular mechanisms underlying low-temperature responses in Pennisetum purpu-reum and offers potential candidate genes for the breeding of cold-tolerant forage grasses.关键词
象草/ERF转录因子/低温胁迫/基因表达分析Key words
Pennisetum purpureum/ERF transcription factor/Low-temperature stress/Gene expression analysis分类
农业科技引用本文复制引用
杨玉娇,贺朝鑫,周洁,李林菊,张新全,彭燕..象草ERF基因家族鉴定及其对低温胁迫的响应分析[J].草地学报,2026,34(9):3223-3233,11.
