草地学报2026,Vol.34Issue(9):3246-3259,14.DOI:10.11733/j.issn.1007-0435.2026.09.010
48份狼尾草属材料表型及遗传多样性分析
Phenotypic and Genetic Diversity Analysis of 48 Pennisetum Germplasm Resources
摘要
Abstract
The features of ecological restoration,ornamental garden,and forage values are possessed by pennis-etum plants.Research on the genetic diversity of pennisetum germplasm resources can be conducted to offer theoretical basis for new variety breeding,development,and utilization.In this study,48 Pennisetum germ-plasm resources were used as materials.Through phenotypic trait determination and SSR molecular marker technology,phenotypic characteristics,genetic diversity,and population structure were systematically evalu-ated.The results showed that the coefficient of variation for four phenotypic traits was observed to range from 28.10%to 96.92%,with a genetic diversity index of 2.49 to 2.83,indicating rich diversity.Plant height was found to be significantly positively correlated with leaf width,while tillering was observed to be significantly negatively correlated with plant height and significantly negatively correlated with leaf width.Phenotypic clus-tering was determined to be divisible into three categories.A total of 122 alleles were detected from 20 primer pairs by SSR markers,with an average of 6.10 per pair.Shannon's diversity index was measured to range from 0.35 to 0.64,polymorphism information content was measured to range from 0.25 to 0.79,and the aver-age genetic distance was calculated to be 0.43.UPGMA clustering was divided into three to five categories as the similarity coefficient was increased,and the population genetic structure was determined to be divisible into three groups with significant differences in genetic background among groups.In this study,the genetic diver-sity and population structure of 48 Pennisetum germplasm resources were elucidated,and a theoretical founda-tion was laid for subsequent germplasm identification and new variety breeding.关键词
狼尾草属/表型多样性/SSR分子标记/遗传多样性/群体结构/DNA指纹图谱Key words
Pennisetum spp./Phenotypic diversity/SSR marker/Genetic diversity/Population analysis/DNA fingerprinting分类
农业科技引用本文复制引用
王伟伟,王豫婉,郭一荻,范希峰,岳跃森,滕珂,孙鑫博,刘凌云..48份狼尾草属材料表型及遗传多样性分析[J].草地学报,2026,34(9):3246-3259,14.基金项目
国家自然科学基金项目(32471755) (32471755)
北京市农林科学院科技创新专项(KJCX20251208) (KJCX20251208)
云南省重点研发计划(202403AP140045)资助 (202403AP140045)