重庆医学2026，Vol.55Issue(8)：1722-1727,6.DOI:10.3969/j.issn.1671-8348.2026.08.007
ACAT1基因干扰对H2O2诱导的RPE细胞氧化应激模型糖代谢及细胞活性、迁移的影响机制
Mechanism of ACAT1 gene interference on glucose metabolism,cell viability and migration of oxidative stress model of RPE cells induced by H2O2
摘要
Abstract
Objective To observe the effects of acetyl-CoA cholesterol acyltransferase 1(ACAT1)gene interference on glucose metabolism,cell viability and migration of hydrogen peroxide(H2O2)-induced oxida-tive stress model of retinal pigment epithelial(RPE)cells,and to explore the potential underlying mecha-nism.Methods Untreated normal human RPE cell line ARPE-19 cells were set as blank group.RPE cells on-ly induced by H2O2 served as induction group.H2O2-induced RPE cells transfected with ACAT1-siRNA were assigned to interference group.Six hours after ACAT1-siRNA transfection,5-adenosine monophosphate-acti-vated protein kinase(AMPK)pathway inhibitor compound C was added to H2O2-induced RPE cells for 6-hour treatment,which was defined as interference+compound C group.At 48 h after treatment,glucose-me-tabolism-related indicators were detected by commercial kits.MTT assay and Transwell assay were used to determine cell survival rate and migration capacity.Western blot was performed to detect the protein expres-sion levels of AMPK,p-AMPK,mammalian target of rapamycin(mTOR)and p-mTOR.Results Compared with the blank group,the induction group exhibited elevated adenosine triphosphate(ATP)level,glucose con-sumption and lactate production,as well as decreased cell survival rate and reduced number of transmembrane cells,with statistically significant differences(P<0.05).Compared with the induction group,the interference group showed increased ATP level,glucose consumption,lactate production,higher cell survival rate and more transmembrane cells(P<0.05).Compared with the interference group,the interference+compound C group presented decreased ATP level,glucose consumption,lactate production,lower cell survival rate and fewer transmembrane cells(P<0.05).The ratio of p-AMPK/AMPK was higher and p-mTOR/mTOR was lower in the induction group relative to the blank group(P<0.05).Compared with the induction group,the interfer-ence group had increased p-AMPK/AMPK(P<0.05)and decreased p-mTOR/mTOR(P<0.05).In con-trast to the interference group,the interference+compound C group displayed reduced p-AMPK/AMPK(P<0.05)and elevated p-mTOR/mTOR(P<0.05).Conclusion ACAT1 gene interference can promote glucose metabolism,cell viability and migration in H2O2-injured RPE cells,accompanied by alterations in the AMPK/mTOR signaling pathway.Compound C intervention can partially reverse these above effects,suggesting that this pathway may be involved in this process.However,rigorous genetic experiments are still required to fur-ther validate its exact mechanism.关键词
乙酰辅酶A胆固醇酰基转移酶1/过氧化氢/视网膜/氧化应激/糖代谢Key words
acetyl-CoA cholesterol acyltransferase 1/hydrogen peroxide/retina/oxidative stress/glu-cose metabolism分类
医药卫生引用本文复制引用
李笑寒,盛小红,王利明,雷博圆,辛向阳..ACAT1基因干扰对H2O2诱导的RPE细胞氧化应激模型糖代谢及细胞活性、迁移的影响机制[J].重庆医学,2026,55(8):1722-1727,6.基金项目
内蒙古自治区自然科学基金项目(2020MS08094) （2020MS08094）
内蒙古自治区科技创新引导项目(CXYD2021BT05) （CXYD2021BT05）
包头市卫生健康科技计划(wsjkkj2022091). （wsjkkj2022091）
