草食家畜Issue(3)：22-29,8.DOI:10.16863/j.cnki.1003-6377.2026.03.004
新疆某羊场流产衣原体流行现状及其主要外膜蛋白结构分析
Prevalence of Chlamydia abortus in a Sheep Farm in Xinjiang and Structural Analysis of Its Major Outer Membrane Protein
摘要
Abstract
[Objective]This study aimed to investigate the prevalence of Chlamydia abortus(C.abortus)in a sheep farm in Xinjiang,elucidate the genetic evolutionary characteristics of its major outer membrane protein(MOMP),and conduct bioinformatics analysis,thereby providing data support for the epidemiological investigation,prevention and control of sheep chlamydiosis.[Methods]A total of 102 samples were collected,including rectal swabs from rams and vaginal swabs from ewes.The MOMP gene of C.abortus was amplified by PCR and sequenced after extraction of genomic DNA.Then sequence alignment,phylogenetic tree construction and relevant bioinformatics analysis were carried out.[Results]The results showed that the positive rate of C.abortus was 1.96%(2/102),which indicated a sporadic infection status at present.The amplified MOMP gene(GenBank accession number PX585115.1)with a total length of 1 170 bp exhibited 100.00%sequence identity with the reference sequences(GenBank accession numbers CP021996.1 and AJ440239.1).Phylogenetic analysis revealed that the detected strain clustered into the same branch as C.abortus,and was clearly distinct from Chlamydia psittaci.Bioinformatics analysis showed that the MOMP protein had a molecular weight of 41.853 79 kDa,a theoretical isoelectric point of 7.54,and an instability index of 20.65,indicating that it was a stable protein without transmembrane domain.The composition of hydrophilic and hydrophobic amino acids in the MOMP protein was well-balanced.The most hydrophilic residues were identified as T99 and P100,while the most hydrophobic residues were N153 and V357.The secondary structure was composed of α-helix(32.13%),random coil(41.65%),extended strand(22.11%),and β-turn(4.11%).[Conclusion]C.abortus was sporadically distributed on a sheep farm in Xinjiang.MOMP is a stable non-transmembrane protein with balanced hydrophilic and hydrophobic amino acids.The MOMP gene sequence of sheep C.abortus in Xinjiang was successfully obtained for the first time by this study,which supplemented basic research data on ovine C.abortus in Xinjiang,and provided reference data for immunological research,epidemiological investigation and scientific prevention and control of the disease.关键词
羊/衣原体/新疆/遗传进化Key words
sheep/Chlamydia abortus/Xinjiang/genetic evolution分类
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郭厚均,苗书魁,米晓云,魏婕,海力且木·买买提依明,党娜娜,丁剑,魏玉荣..新疆某羊场流产衣原体流行现状及其主要外膜蛋白结构分析[J].草食家畜,2026,(3):22-29,8.基金项目
新疆维吾尔自治区"三农"骨干人才培养项目(2024SNGGNT060) （2024SNGGNT060）
新疆维吾尔自治区重点研发专项(2025B02022-1) （2025B02022-1）
