茶叶通讯2026，Vol.53Issue(3)：315-327,13.DOI:10.20178/j.cnki.ISSN1009-525X.2026.03.005
珍贵植物'疏齿大厂茶'叶绿体基因组分析
Chloroplast Genome Analysis of the Rare Plant Camellia tachangensis var.remotiserrata
摘要
Abstract
To reveal the chloroplast genome characteristics and phylogenetic relationships of Camellia tachangensis var remotiserrata,an economically and ecologically valuable plant in northern Guizhou,its chloroplast genome was sequenced,assembled,and analyzed using high-throughput sequencing technology and bioinformatic methods,with comparisons made to six other Camellia species.The results showed that the chloroplast genome of Camellia tachangensis var.remotiserrata exhibited a typical quadripartite structure,with a genome size of 156,883 bp and GC content of 37.33%.It encoded 37 tRNA genes,8 rRNA genes,89 protein-coding genes,and one pseudogene.Analysis of codon usage bias in protein-coding genes revealed 31 codons with a relative synonymous codon usage(RSCU)value>1,indicating a preference for A/U at the third codon position.Simple sequence repeat(SSR)analysis detected 244 SSR loci,predominantly composed of A/T bases.Analysis of dispersed repeats identified only two types:forward repeats(16)and palindromic repeats(22);no reverse or complementary repeats were found.Screening for nucleotide diversity(Pi)identified four genes(psbM with Pi=0.0054,rps12 with Pi=0.008,rpl16 with Pi=0.0063,and rps19 with Pi=0.0068)containing highly variable regions.The Ka/Ks ratios for the matK and rpoB genes in some Camellia species were greater than 1,suggesting positive selection acting on these genes.Comparative analysis of inverted repeat(IR)boundaries showed they were conserved between Camellia tachangensis var remotiserrata and the six other Camellia species,with sequence variations primarily located within the large single-copy(LSC)and small single-copy(SSC)regions.Phylogenetic analysis indicated that Camellia tachangensis var remotiserrata was closely related to Camellia gymnogyna,Camellia danzaiensis,and Camellia costata,but maintained a relatively large genetic distance from Camellia tachangensis.This study provided fundamental data for phylogenetic analysis within Camellia,species identification,and the development of DNA barcodes for these plants.关键词
茶树/'疏齿大厂茶'/叶绿体/基因组/结构特征/系统发育Key words
Tea plant/Camellia tachangensis var.remotiserrata/Chloroplast/genome/Structural characterization/Phylogeny分类
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王逍,罗康,徐建,周美,熊玲,穆君..珍贵植物'疏齿大厂茶'叶绿体基因组分析[J].茶叶通讯,2026,53(3):315-327,13.基金项目
中央国家级自然保护区补助资金项目 （）
