茶叶通讯2026，Vol.53Issue(3)：357-366,10.DOI:10.20178/j.cnki.ISSN1009-525X.2026.03.009
指纹图谱结合化学计量学评价菊花决明子茶的质量
Quality Evaluation of Chrysanthemum and Cassia Seed Tea by Fingerprint Combined with Chemometrics
摘要
Abstract
This study aimed to establish a high-performance liquid chromatography(HPLC)fingerprint for chrysanthemum and cassia seed tea,simultaneously determine the contents of 13 marker components,and combine chemometric analysis to evaluate the quality consistency of different batches,thereby providing technical support for the quality control of this health tea.The analysis was performed on a Zafex Supfex AQ-C18 column(250 mm×4.6 mm,5 µm)with a mobile phase of acetonitrile and 0.3%phosphoric acid solution using a binary low-pressure gradient elution program.The flow rate was 0.8 mL/min,the column temperature was maintained at 30℃,and the detection wavelength was set at 254 nm.The similarity of chromatograms from 20 batches of samples was evaluated using the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 version).Cluster analysis(CA)and principal component analysis(PCA)were conducted using SPSS software.The contents of 13 common components—namely chlorogenic acid,caffeic acid,ferulic acid,verbascoside,luteoloside,rutin,3,5-O-dicaffeoylquinic acid,arctiin,4,5-O-dicaffeoylquinic acid,quercetin,kaempferol,aurantio-obtusin,and chrysophanol—were simultaneously quantified using the external standard method.An HPLC fingerprint method for chrysanthemum and cassia seed tea was successfully established,with 25 common peaks calibrated and 13 of them identified.The 13 components showed good linear relationships within their respective concentration ranges(r2≥0.9619).The average spike recovery rates ranged from 88.33%to 100.28%.Similarity evaluation,CA,and PCA all indicated quality variations among the different batches.The established method was accurate,reliable,and reproducible.The integrated application of chromatographic fingerprinting,multi-component quantification,and chemometric analysis provided a systematic and effective approach for the quality control and comprehensive evaluation of chrysanthemum and cassia seed tea.关键词
菊花决明子茶/高效液相色谱法/指纹图谱/含量测定/化学计量学Key words
Chrysanthemum and cassia seed tea/High-performance liquid chromatography(HPLC)/Fingerprint/Content determination/Chemometrics分类
轻工纺织引用本文复制引用
段爱莉,庞文娟,田小琴,杨生梅,秦海钰,董斌,孙东德..指纹图谱结合化学计量学评价菊花决明子茶的质量[J].茶叶通讯,2026,53(3):357-366,10.基金项目
武威市2024年度市级科技计划项目(WW24A01QYJ006),武威市2024年度市级科技计划自筹经费项目(WW24B01GY005),武威市 2025年度市级科技计划民生科技计划项目(WW25A03MSX006) （WW24A01QYJ006）
