茶叶通讯2026，Vol.53Issue(3)：367-378,12.DOI:10.20178/j.cnki.ISSN1009-525X.2026.03.010
不同茶树品种多酚氧化酶体外酶促合成聚酯型儿茶素研究
Synthesis of Theasinensins by In Vitro Enzymatic Oxidation Using Polyphenol Oxidase from Different Tea Cultivars
摘要
Abstract
Using crude polyphenol oxidase(PPO)extracts prepared from the summer one-bud-one-leaf fresh leaves of Baojing Huangjincha 1,Zhuyeqi,Bixiangzao,and Taoyuan Daye as enzyme sources and instant green tea as the substrate,this study optimized parameters for in vitro enzymatic oxidation through single-factor and orthogonal experiments to investigate the effects of PPO from different tea cultivars on the in vitro enzymatic synthesis of theasinensins.The results showed that the optimal parameters for the enzyme sources from Baojing Huangjincha 1,Bixiangzao,Zhuyeqi,and Taoyuan Daye were as follows:pH 6.2,enzyme source volume 1.5 mL,reaction temperature 35.0℃,and reaction time 180 min;pH 5.8,enzyme source volume 1.3 mL,reaction temperature 27.5℃,and reaction time 120 min;pH 6.2,enzyme source volume 1.1 mL,reaction temperature 27.5℃,and reaction time 135 min;and pH 6.2,enzyme source volume 1.3 mL,reaction temperature 25℃,and reaction time 165 min.Under its optimal parameters,the enzyme source from'Baojing Huangjincha'achieved a theasinensins content of 2.91 μg·mL-1,making it the most effective enzyme source among the four cultivars tested.关键词
茶树品种/多酚氧化酶/酶促合成/聚酯型儿茶素Key words
Tea cultivar/Polyphenol oxidase/Enzymatic synthesis/Theasinensins分类
农业科技引用本文复制引用
罗依斯,田金玺,刘梦圆,肖文军..不同茶树品种多酚氧化酶体外酶促合成聚酯型儿茶素研究[J].茶叶通讯,2026,53(3):367-378,12.基金项目
湖南省教育科学"十四五"规划课题(XJK25CGD073),国家重点研发计划项目(2022YFD1600805),湖南省芙蓉计划科技领军人才项目(2024) （XJK25CGD073）
