东北林业大学学报2026，Vol.54Issue(10)：10-15,40,7.
核-壳型磁性纳米颗粒的制备及其在生物育种中的应用
Preparation of Core-Shell-Structured Magnetic Nanoparticles and Their Applications in Biological Breeding
摘要
Abstract
Silicon-hydroxyl magnetic nanoparticles were prepared via a ligand-exchange method on the basis of a solvothermal route,followed by surface modification and functional alteration.The successfully synthesized nanoparticles possess high specific surface area,high surface energy and uniform particle size,which endows them with high water-solubility and fa-vorable biocompatibility simultaneously.In biological-breeding-related experiments,silicon hydroxyl magnetic nanoparti-cles show outstanding performance.They can efficiently isolate pBI121-BpCDF3-GFP plasmid DNA and are applicable to extract high-quality genomic DNA and total RNA from transgenic BpCDF3 materials.The established extraction method presents prominent advantages in plant genomic DNA,RNA and target gene plasmids.It shortens operation time and im-proves nucleic-acid concentration and purity,which provides a reliable technical support for subsequent molecular-biology experiments and supplies innovative materials and solutions for streamlining the experimental procedures of biotechnology-based breeding.关键词
磁性纳米颗粒/功能化磁珠/生物育种/核酸提取Key words
Magnetic nanoparticles/Functionalized magnetic beads/Biological breeding/Nucleic acid extraction分类
农业科技引用本文复制引用
宋宇露,王丽娜..核-壳型磁性纳米颗粒的制备及其在生物育种中的应用[J].东北林业大学学报,2026,54(10):10-15,40,7.基金项目
中央高校基本科研业务费专项资金项目(2572022DS02) （2572022DS02）
林木遗传育种全国重点实验室创新项目(2024B01). （2024B01）
