东北林业大学学报2026，Vol.54Issue(10)：27-40,14.
油茶BAM基因家族的鉴定及低温胁迫下表达模式
Identification of the BAM Gene Family in Camellia oleifera and Its Expression Patterns under Low-Temperature Stress
摘要
Abstract
β-amylase(BAM)plays a pivotal role in breaking down starch into soluble sugars and directly participates in low-temperature stress resistance in plants.Based on the genome data of Camellia oleifera Abel.,we systematically identified the CoBAMs(BAM gene family of C.oleifera)via bioinformatic approaches,analyzed their evolutionary and regulatory characteristics,and explored their expression patterns under different low-temperatures(-6,-2,and 2℃)and cold-treatment durations(6,12,and 24 h),to uncover the potential functions of this gene family during cold-stress resistance in C.oleifera.The results showed that 10 CoBAMs genes were identified from the C.oleifera genome and unevenly distrib-uted across four chromosomes,and most encoded proteins were localized to the nucleus or chloroplast.Phylogenetic analy-sis classified these CoBAMs into three clades,all containing a conserved Glyco_hydro_14 domain,genes within the same clade exhibited highly conserved gene structures and motif compositions.Collinearity analysis revealed intra-genomic gene duplications for the CoBAMs family,and collinear relationships were detected between CoBAMs and BAM genes from close-ly-related species.Prediction of promoter cis-acting elements and upstream transcription factors indicated that CoBAMs pro-moters harbored abundant stress-responsive elements,and their expression was potentially modulated by ERF,Dof,BBR-BPC,C2H2 and other transcription factors.RT-qPCR results demonstrated that the transcript levels of most CoBAMs genes were markedly elevated in leaves.Under low-temperature treatments,most CoBAMs were significantly induced yet displayed distinct expression patterns under different cold conditions.Notably,the expression level of CoBAM8 continuous-ly increased with prolonged cold-treatment time regardless of temperature,implying that it acts as a key gene for cold-stress response.Moreover,β-amylase activity and soluble-sugar contents increased significantly under cold conditions,further supporting the essential roles of CoBAMs in cold resistance of C.oleifera.关键词
油茶/BAM家族/生物信息学分析/表达模式分析/低温胁迫Key words
Camellia oleifera/BAM family/Bioinformatic analysis/Expression pattern analysis/Low-temperature stress分类
农业科技引用本文复制引用
郑烨,周嘉翼,王琪,刘彩霞,夏建梅,孙永江..油茶BAM基因家族的鉴定及低温胁迫下表达模式[J].东北林业大学学报,2026,54(10):27-40,14.基金项目
岳麓山实验室种业专项项目(YLS-2025-ZY04045) （YLS-2025-ZY04045）
国家自然科学基金项目(32001348). （32001348）
