大连工业大学学报2026，Vol.45Issue(4)：235-240,6.DOI:10.19670/j.cnki.dlgydxxb.2026.0401
人参主根与须根总皂苷的提取、纯化及其活性
Extraction,purification and activities of total ginsenosides from ginseng roots and root fibers
摘要
Abstract
Total saponins from the taproots and fibrous roots of Panax ginseng were extracted and purified by a combined impregnation-macroporous resin technique,and their component characteristics were analyzed by HPLC.The antibacterial effects against Escherichia coli(Gram-negative bacterium)and Bacillus subtilis(Gram-positive bacterium)were evaluated by the disk diffusion method and the microdilution method,while the mechanism of membrane potential change was elucidated by the Rhodamine 123 fluorescence method.The antioxidant activity profiles of the two root types were systematically compared using the ABTS+radical scavenging assay.The results showed that the taproot saponins were predominantly composed of Rg1(32.45％)and Rb1(27.95％),whereas the fibrous roots were enriched in Rb1(31.17％)and Rc(14.42％).Significant inhibition against E.coli was exhibited by the total saponins(MIC=0.6 mg/mL),while a higher concentration was required for the suppression of B.subtilis.Among the individual saponins,the strongest antibacterial activity against E.coliwasdisplayed by R1,Rb1,Rc,and Rd(inhibition zone 10.2 to 12.4 mm),and effectiveness against B.subtilis was shown by Rg1,Rb1,and Rc.Membrane depolarization was induced and ATP synthesis was interfered with by the saponins,whereby the antibacterial effect was achieved.Certain antioxidant activities were demonstrated by both taproot and fibrous root saponins,and a superior ABTS+scavenging capacity was observed for the fibrous root saponins compared to the taproot ones.It was concluded that differences in chemical composition,antibacterial and antioxidant properties existed between the taproot and fibrous root saponins of P.ginseng,with stronger antibacterial efficacy exhibited by the taproots and better antioxidant activity shown by the fibrous roots.关键词
人参主根皂苷/人参须根皂苷/大肠杆菌/枯草芽孢杆菌/抑菌效果/抗氧化Key words
ginseng root ginsenosides/ginseng root fibers ginsenosides/Escherichia coli/Bacillus subtilis/antibacterial effects/antioxidant activity分类
医药卫生引用本文复制引用
徐丝瑜,马梦晓,陈丽媛,徐子一,屈志航,鱼红闪..人参主根与须根总皂苷的提取、纯化及其活性[J].大连工业大学学报,2026,45(4):235-240,6.基金项目
辽宁省教育厅基本科研项目(JYTQN2023116) （JYTQN2023116）
辽宁省高校基本科研业务费(LJBKY2024050). （LJBKY2024050）
