大连工业大学学报2026，Vol.45Issue(4)：241-246,6.DOI:10.19670/j.cnki.dlgydxxb.2026.0402
长春花CrLAMT基因促进喜树中喜树碱的生物合成
The CrLAMT gene in Catharanthus roseus promotes the biosynthesis of camptothecin in Camptotheca acuminata
摘要
Abstract
Camptothecin(CPT),a monoterpene indole alkaloid with notable anticancer properties,is produced in Camptotheca acuminata.Due to the absence of methylated intermediates in this plant,its biosynthetic pathway is solely reliant on carboxylic acid intermediates;whereas in Catharanthus roseus,the monoterpene indole alkaloid(MIA)pathway is characterized by the high expression of the methyltransferase LAMT and the synthesis of methylated intermediates,which enables efficient MIA production.To improve CPT yield,the loganic acid O-methyltransferase from C.roseus was heterologously expressed in C.acuminata.It was demonstrated that the overexpression of CrLAMT significantly enhanced downstream CPT production through the synthesis of the methylated intermediate loganin.The introduced CrLAMT was confirmed to successfully initiate the methylated intermediate pathway in C.acuminata based on the original carboxylic acid pathway,by which loganic acid was converted into loganin.The synergistic action of the two metabolic pathways was shown to markedly increase the CPT content in C.acuminata.关键词
CrLAMT基因/喜树碱/生物合成途径/代谢支路Key words
gene CrLAMT/camptothecin/biosynthesis pathway/metabolic branch分类
农业科技引用本文复制引用
黄茹,马文慧,杨秉润,苏千祎,寇萍..长春花CrLAMT基因促进喜树中喜树碱的生物合成[J].大连工业大学学报,2026,45(4):241-246,6.基金项目
辽宁省教育厅基本科研项目(JYTQN2023110). （JYTQN2023110）
