大连工业大学学报2026，Vol.45Issue(4)：247-251,5.DOI:10.19670/j.cnki.dlgydxxb.2026.0403
糖浓度和溶解氧协同控制提高红法夫酵母的发酵生物量
Synergistic control of sugar concentration and dissolved oxygen to improve the fermentation biomass of Phaffia rhodozyma
摘要
Abstract
The high-density fermentation conditions of P.rhodozyma were optimized in a 100 L fermenter.During fermentation,the dissolved oxygen coefficient in the broth was controlled by feeding low-concentration sugar,to enhance biomass.The optimal conditions were as follows:glucose concentration maintained at 15.0 to 20.0 g/L by controlling sugar feeding rate,pH adjusted to 4.8 to 5.3 with ammonia water,and dissolved oxygen stabilized at 15.0％to 20.0％.After 64 h fermentation,wet and dry biomass reached 288.5 g/L and 77.2 g/L,respectively.Colorimetry and HPLC results showed that total carotenoid content in dry biomass was 14.6 mg/g,while astaxanthin content was 523.2 μg/g,and astaxanthin in broth was 40.4 mg/L.关键词
红法夫酵母/虾青素/发酵生物量/过程控制Key words
Phaffia rhodozyma/astaxanthin/fermentation biomass/process control分类
生物科学引用本文复制引用
丛睿林,浦军平,张洁霖,严明凯,付冬梅..糖浓度和溶解氧协同控制提高红法夫酵母的发酵生物量[J].大连工业大学学报,2026,45(4):247-251,5.基金项目
辽宁省教育厅高等学校基本科研项目(LJKZ0516). （LJKZ0516）
