电子科技2026，Vol.39Issue(9)：51-57,7.DOI:10.16180/j.cnki.issn1007-7820.2026.09.007
野外实时PCR控制系统的设计
Design of A Real-Time PCR Control System for Field Use
摘要
Abstract
In order to improve the performance of traditional PCR(Polymerase Chain Reaction)equipment in terms of portability,shock resistance and efficiency,a real-time PCR control system suitable for field environments is designed based on the PCR principle and embedded control technology.The key components are installed in the shock-absorbing frame by adopting a suspended structure,and the shell is made of high-temperature resistant resin material.Lightweighting and high-temperature resistance are achieved through 3D printing technology.Build a high-precision temperature control system with the help of TEC(Thermoelectric Cooler).The system integrates re-al-time detection functions and realizes dynamic monitoring of the amplification process through motor-driven fluo-rescence detectors.Amplification experiments are conducted on inactivated hepatitis B virus samples on this de-vice,achieving high-precision temperature control of±0.1℃,with a heating rate of 3.2℃·s-1 and a cooling rate of 4.5℃·s-1.This reduced the single PCR cycle time to 110 s and displayed the amplification process in real time on the screen.The proposed system not only enhances the efficiency and stability of PCR operations,but also pro-vides a new technical solution for mobile PCR detection.关键词
荧光PCR/嵌入式/荧光检测/实时检测/温度控制/TEC/乙肝病毒/野外Key words
fluorescence PCR/embedded/fluorescence detection/real-time detection/temperature control/TEC/hepatitis B virus/field分类
信息技术与安全科学引用本文复制引用
焦世纪,瑚琦,徐志凯..野外实时PCR控制系统的设计[J].电子科技,2026,39(9):51-57,7.基金项目
国家自然科学基金(62474112)National Natural Science Foundation of China(62474112) （62474112）
