福建农林大学学报（自然科学版）2026，Vol.55Issue(5)：652-661,10.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202604002
单细胞转录组测序技术在植物研究中的应用
Application of single-cell sequencing technologies in plant research
摘要
Abstract
[Background]Single-cell RNA sequencing(scRNA-seq)enables the analysis of gene expression dynamics and cellular heterogeneity in plants at single-cell resolution,addressing the limitations of bulk transcriptomics in cell-type resolution.As a direct readout of cellular states,scRNA-seq has become an essential tool for studying plant development and cell differentiation.[Pro-gress]Recent advances in sample preparation,sequencing strategies and computational analysis have broadened its application to di-verse tissues and species.High-resolution single-cell atlases have unveiled cell differentiation trajectories and regulatory networks,deepening our understandings of organ development,abiotic stress responses and plant-pathogen interactions.However,technical bottlenecks remain,which include inefficient cell isolation,limited capture of certain cell types and lack of spatial imformation.[Prospect]Future integration with single-nucleus sequencing,multi-omics approaches and spatial transcriptomics is expected to pro-vide a more comprehensive panorama of cellular states and their regulatory networks,providing fundamental support for precise crop improvement and stress-resistance mechanism studies.关键词
单细胞转录组测序/基因表达/细胞异质性/植物生长发育/生物胁迫/非生物胁迫Key words
single-cell RNA sequencing/gene expression/cellular heterogeneity/plant growth and development/biotic stress/abiotic stress分类
农业科技引用本文复制引用
李君鹏,安雯霞,吴建国..单细胞转录组测序技术在植物研究中的应用[J].福建农林大学学报（自然科学版）,2026,55(5):652-661,10.基金项目
农林生物安全全国重点实验室联合攻关项目(SKLJRP2504). （SKLJRP2504）
