福建农林大学学报（自然科学版）2026，Vol.55Issue(5)：708-716,9.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202509008
玉米生平脐蠕孢菌的产黄青霉病毒鉴定及其对寄主生长和致病性的影响
Identification of a chrysovirus in Bipolaris zeicola and its impact on host growth and pathogenicity
摘要
Abstract
[Objective]The diversity of mycovirus in Bipolaris zeicola was investigated to provide theoretical support for the ecological control of northern corn leaf pot.[Method]Double-stranded RNA(dsRNA)was extracted from B.zeicola strain CY56,followed by sequencing and identification of the mycovirus.Biological experiments were further conducted to evaluate the effects of the mycovirus on the growth and pathogenicity of strain CY56.[Result]Four dsRNA segments were isolated from CY56,and identified as a novel chrysovirus through sequence analysis,named Bipolaris zeicola chrysovirus 1(BzCV1).BzCV1 belonged to the genus Betachrysovir-us within Chrysoviridae,and exhibited high phylogenetic homology with Curvularia lunata chrysovirus 1(CuCV1),Alternaria alter-nate chrysovirus 1(AaCV1),Setosphaeria turcica chrysovirus 1(StCV1),and Bipolaris maydis chrysovirus 2(BmCV2),with RNA dependent RNA polymerase(RdRP)sequence identities of 75.6%,79.4%,84.5%and 87.9%,respectively.Biological ex-periments showed no significant differences in growth characteristics or pathogenicity between CY56 and the virus-free control strain CY01.[Conclusion]A chrysovirus was first indentified from B.zeicola,which exterted insignificant effect on the growth and pathoge-nicity of its host.关键词
真菌病毒/产黄青霉病毒/双链 RNA/玉米生平脐蠕孢菌/致病性Key words
mycovirus/chrysovirus/double-stranded RNA/Bipolaris zeicola/pathogenicity分类
农业科技引用本文复制引用
陈苗苗,刘卫容,郑郧,杜振国,贾东升,张鑫,彭迪,王有宁,章松柏..玉米生平脐蠕孢菌的产黄青霉病毒鉴定及其对寄主生长和致病性的影响[J].福建农林大学学报（自然科学版）,2026,55(5):708-716,9.基金项目
国家自然科学基金项目(31972243) （31972243）
湖北省自然科学基金项目(2024AFB1015) （2024AFB1015）
特色作物资源利用与品质控制湖北省重点实验室开放基金项目(2024K006). （2024K006）
