福建农业学报2026，Vol.41Issue(6)：662-672,11.DOI:10.19303/j.issn.1008-0384.2026.06.003
基于蛋白组学分析不同裂叶类型五指毛桃黄酮类化合物的差异
Flavonoid-related Proteomic Variations in Different Types of Ficus Hirta Vahl.
摘要
Abstract
[Objective]Total flavonoid,antioxidant capacity,and differentially expressed proteins(DEPs)associated with flavonoids accumulation in the roots of different types of Ficus hirta were determined.[Method]Roots of 3-year-old,5-lobed(FF),7-lobed(SF),and 9-lobed(NF)F.hirta Vahl.were collected for the determinations of flavonoid content and antioxidant activity as well as flavonoid synthesis-related DEP expressions by a proteomic analysis.[Result]The total flavonoid content and antioxidant capacity of NF were significantly higher than those of the other two types.As indicated by the KEGG analysis,the DEPs were significantly enriched in phenylpropanoid and flavonoid synthesis pathways.Thirty-two key enzymes closely related to flavonoid synthesis were identified.They included the ones mostly upregulated in NF,such as phenylalanine ammonia-lyase(PAL),4-coumarate-CoA ligase(4CL),chalcone synthase(CHS),chalcone isomerase(CHI),and caffeic acid O-methyltransferase(COMT).Extensive synergistic interactions existed among these enzymes to jointly regulate the flavonoid synthesis in the plants.[Conclusion]The DEPs associated with flavonoid synthesis in the different F.hirta Vahl.were identified at molecular level.The synergistic effect of the up-regulated key enzymes in the phenylpropanoid and flavonoid branch pathways appeared to result in the high flavonoid accumulation of NF variety.关键词
五指毛桃/裂叶类型/蛋白质组学/差异表达蛋白/类黄酮生物合成/液质联用Key words
Ficus hirta Vahl/lobe type/proteomics/differential expressed proteins/flavonoid biosynthesis/liquid chromatography-mass spectrometry分类
农业科技引用本文复制引用
陈荣珠,王小平,林鹭萍,陈汉鑫,马馨怡,赖钟雄..基于蛋白组学分析不同裂叶类型五指毛桃黄酮类化合物的差异[J].福建农业学报,2026,41(6):662-672,11.基金项目
福建省科技计划引导性项目(2023N0054) （2023N0054）
第三批国家级职业教育教师创新团队项目(教师函[2023]9号) （教师函[2023]9号）
漳州卫生职业学院课题(ZWYCX202404) （ZWYCX202404）
